A MALDI-TOF Mass Spectrometry Approach to Investigate the Defense Reactions
173
Caprioli R, Farmer T, Gile 1 (1997) Molecular imaging of biological samples: localization of pep tides
and proteins using MALDI-TOF MS. Anal Chern 69: 4751-4760
Chalfie M, Tu Y, Euskirchen G, Ward W W, Prasher D C (1994) Green Fluorescent Protein as a marker
for gene expression. Science 263: 802-805
Charlet M, Lagheux M, Reichhart 1M, Hoffmann lA (1996) Cloning of the gene encoding the antibacterial peptide drosocin involved in Drosophila immunity. Expression studies during the immune
response. Eur 1 Biochem 241: 699 - 706
Chosa N, Fukumitsu T, Fujimoto K, Ohnishi E (1997) Activation of prophenoloxidase Al by an activating enzyme in Drosophila melanogaster. Insect Biochem Mol Bioi 27: 61-68
Claydon M, Davey S, Edwards-Jones V, Gordon D (1996) The rapid identification of intact microorganisms using mass spectrometry. Nat Biotech 14: 1584-1586
Cohen S, Chait B (1996) Influence of matrix solution conditions on the MALDI-MS analysis of peptides and proteins. Anal Chern 68: 31-37
Dimarcq J L, Hoffmann D, Meister M, Bulet P, Lanot R, Reichhart J M, Hoffmann J A (1994) Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. A study in
insect immunity. Eur J Biochem 221: 201- 209
Easterling M, Colangelo C, Scott R, Amster I (1998) Monitoring protein expression in whole bacterial
cells with MALDI time-of-flight mass spectrometry. Anal Chern 70: 2704-2709
Ezekowitz R, Hoffmann J (1998) Innate immunity. The blossoming of innate immunity. Curr Opin
ImmunollO: 9-11
Fearon D, Locksley R (1996) The instructive role of innate immunity in the acquired immune
response. Science 272: 50-53
Fehlbaum P, Bulet P, Michaut L, Lagueux M, Broekaert W, Hetru C, Hoffmann J (1994) Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence
homology to plant antifungal peptides. 1 Bioi Chern 269: 33159-33163
Ferrandon D, lung A C, Criqui M, Lemaitre B, Uttenweiler-Joseph S, Michaut L, Reichhart 1, Hoffmann J A (1998) A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. EMBO J 17: 1217-1227
Franc N C, Dimarcq J L, Lagueux M, Hoffmann J A, Ezekowitz A B (1996) Croquemort, a novel Drosophila hemocyte/macrophage receptor that recognizes apoptotic cells. Immunity 4: 434-444
Fujimoto K, Masuda K, Asada N, Ohnishi E (1993) Purification and characterization of prop he nolo xidases from pupae of Drosophila melanogaster. J Biochem 113: 285 - 291
Hoffmann J, Reichhart J (1997) Drosophila immunity. Trends in Cell Bioi 7: 309-316
Jensen 0 N, Mortensen P, Vorm 0, Mann N (1997) Automation of matrix assisted laser desorption/
ionization-mass spectrometry using fuzzy logic feedback control. Anal Chern 69: 1706-1714
Jimenez C, van Veelen P, Li K, Wildering W, Geraerts W, Tjaden U, van der GreefJ (1994) Neuropeptide expression and processing as revealed by direct matrix-assisted laser desorption/ionization
mass spectrometry of single neurons. 1 Neurochem 62: 404-407
Kratzer R, Eckerskorn C, Karas M, Lottspeich F (1998) Suppression effects in enzymatic peptide ladder sequencing using ultraviolet-matrix assisted laser desorption/ionization mass spectrometry.
Electrophoresis 19: 1910-1919
Kussmann M, Nordhoff E, Rahbek-Nielsen H, Haebel S, Rossel-Larsen M, Jakobsen L, Gobom J, Mirgorodskaya E, Kroll-Kristensen A, Palm L, Roepstorff P (1997) Matrix-assisted laser desorption/
ionization mass spectrometry sample preparation techniques designed for various pep tides and
protein analytes. J Mass Spectrom 32: 593-601
Landon C, Sodano P, Hetru C, Hoffmann J, Ptak M (1997) Solution structure of drosomycin, the first
inducible antifungal protein from insects. Protein Sci 6: 1878-1884
Lemaitre B, Kromer-Metzger E, Michaut L, Nicolas E, Meister M, Georgel P, Reichhart J, Hoffmann 1
(1995) A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the
Drosophila host defence. Proc Nat! Acad Sci USA 92: 9465-9469
Lemaitre B, Nicolas E, Michaut L, Reichhart J, Hoffmann J (1996) The dorsoventral regulatory gene
cassette spiitzle!Toll!cactus controls the potent antifungal response in Drosophila adults. Cell 86:
973-983
Levashina E, Ohresser S, Bulet P, Reichhart J, Hetru C, Hoffmann J (1995) Metchnikowin, a novel
immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties. Eur J Biochem 233: 694-700
Levashina E A, Ohresser S, Lemaitre B, hnler J L (1998) Two distinct pathways can control expression
of the gene encoding the Drosophila antimicrobial peptide metchnikowin. J Mol Bioi 278: 515-527
Li K W, Hoek R M, Smith F, Jimenez C R, van der Schors R C, van Veelen P A, Chen S, van der Greef
J, Parish D C, Benjamin P R, Geraerts WPM (1994) Direct peptide profiling by mass spectrometry
of single identified neurons reveals complex neuropeptide-processing pattern. J Bioi Chern 269:
30288-30292
173
Caprioli R, Farmer T, Gile 1 (1997) Molecular imaging of biological samples: localization of pep tides
and proteins using MALDI-TOF MS. Anal Chern 69: 4751-4760
Chalfie M, Tu Y, Euskirchen G, Ward W W, Prasher D C (1994) Green Fluorescent Protein as a marker
for gene expression. Science 263: 802-805
Charlet M, Lagheux M, Reichhart 1M, Hoffmann lA (1996) Cloning of the gene encoding the antibacterial peptide drosocin involved in Drosophila immunity. Expression studies during the immune
response. Eur 1 Biochem 241: 699 - 706
Chosa N, Fukumitsu T, Fujimoto K, Ohnishi E (1997) Activation of prophenoloxidase Al by an activating enzyme in Drosophila melanogaster. Insect Biochem Mol Bioi 27: 61-68
Claydon M, Davey S, Edwards-Jones V, Gordon D (1996) The rapid identification of intact microorganisms using mass spectrometry. Nat Biotech 14: 1584-1586
Cohen S, Chait B (1996) Influence of matrix solution conditions on the MALDI-MS analysis of peptides and proteins. Anal Chern 68: 31-37
Dimarcq J L, Hoffmann D, Meister M, Bulet P, Lanot R, Reichhart J M, Hoffmann J A (1994) Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. A study in
insect immunity. Eur J Biochem 221: 201- 209
Easterling M, Colangelo C, Scott R, Amster I (1998) Monitoring protein expression in whole bacterial
cells with MALDI time-of-flight mass spectrometry. Anal Chern 70: 2704-2709
Ezekowitz R, Hoffmann J (1998) Innate immunity. The blossoming of innate immunity. Curr Opin
ImmunollO: 9-11
Fearon D, Locksley R (1996) The instructive role of innate immunity in the acquired immune
response. Science 272: 50-53
Fehlbaum P, Bulet P, Michaut L, Lagueux M, Broekaert W, Hetru C, Hoffmann J (1994) Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence
homology to plant antifungal peptides. 1 Bioi Chern 269: 33159-33163
Ferrandon D, lung A C, Criqui M, Lemaitre B, Uttenweiler-Joseph S, Michaut L, Reichhart 1, Hoffmann J A (1998) A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. EMBO J 17: 1217-1227
Franc N C, Dimarcq J L, Lagueux M, Hoffmann J A, Ezekowitz A B (1996) Croquemort, a novel Drosophila hemocyte/macrophage receptor that recognizes apoptotic cells. Immunity 4: 434-444
Fujimoto K, Masuda K, Asada N, Ohnishi E (1993) Purification and characterization of prop he nolo xidases from pupae of Drosophila melanogaster. J Biochem 113: 285 - 291
Hoffmann J, Reichhart J (1997) Drosophila immunity. Trends in Cell Bioi 7: 309-316
Jensen 0 N, Mortensen P, Vorm 0, Mann N (1997) Automation of matrix assisted laser desorption/
ionization-mass spectrometry using fuzzy logic feedback control. Anal Chern 69: 1706-1714
Jimenez C, van Veelen P, Li K, Wildering W, Geraerts W, Tjaden U, van der GreefJ (1994) Neuropeptide expression and processing as revealed by direct matrix-assisted laser desorption/ionization
mass spectrometry of single neurons. 1 Neurochem 62: 404-407
Kratzer R, Eckerskorn C, Karas M, Lottspeich F (1998) Suppression effects in enzymatic peptide ladder sequencing using ultraviolet-matrix assisted laser desorption/ionization mass spectrometry.
Electrophoresis 19: 1910-1919
Kussmann M, Nordhoff E, Rahbek-Nielsen H, Haebel S, Rossel-Larsen M, Jakobsen L, Gobom J, Mirgorodskaya E, Kroll-Kristensen A, Palm L, Roepstorff P (1997) Matrix-assisted laser desorption/
ionization mass spectrometry sample preparation techniques designed for various pep tides and
protein analytes. J Mass Spectrom 32: 593-601
Landon C, Sodano P, Hetru C, Hoffmann J, Ptak M (1997) Solution structure of drosomycin, the first
inducible antifungal protein from insects. Protein Sci 6: 1878-1884
Lemaitre B, Kromer-Metzger E, Michaut L, Nicolas E, Meister M, Georgel P, Reichhart J, Hoffmann 1
(1995) A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the
Drosophila host defence. Proc Nat! Acad Sci USA 92: 9465-9469
Lemaitre B, Nicolas E, Michaut L, Reichhart J, Hoffmann J (1996) The dorsoventral regulatory gene
cassette spiitzle!Toll!cactus controls the potent antifungal response in Drosophila adults. Cell 86:
973-983
Levashina E, Ohresser S, Bulet P, Reichhart J, Hetru C, Hoffmann J (1995) Metchnikowin, a novel
immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties. Eur J Biochem 233: 694-700
Levashina E A, Ohresser S, Lemaitre B, hnler J L (1998) Two distinct pathways can control expression
of the gene encoding the Drosophila antimicrobial peptide metchnikowin. J Mol Bioi 278: 515-527
Li K W, Hoek R M, Smith F, Jimenez C R, van der Schors R C, van Veelen P A, Chen S, van der Greef
J, Parish D C, Benjamin P R, Geraerts WPM (1994) Direct peptide profiling by mass spectrometry
of single identified neurons reveals complex neuropeptide-processing pattern. J Bioi Chern 269:
30288-30292