Elucidation of Functionally Significant Structural Modifications
a.i.
A
5000
1046.54
4000
1409.83
3000
2048,06
2000
1000
.i
.1
.• J.>.
1
o
5000
B
1976.06
4000
1046.54
3000
1409.81
2000
I
J J
1000
.l
,I.
o
1100
1600
2100
m/z
2465,20
.1
2465.20
125
2731.52
t ,1
2731 .52
,1, J .1.
2600
Fig. 8.4. MALD! maps of AspN protease digests of the F2-polypeptides of (A) the VRI 82-6409 and
(B) V 4 isolates of NDV. Ions at mlz values of 1046.54 and 2465.2 are the monoisotopic ions of the
internal calibrants angiotensin II and ACTH 18-39, respectively. These spectra were all collected
using delayed extraction and a digitization rate of 1 GHz. These spectra were adapted with permission
from (Lopaticki et al. 1998; Copyright, John Wiley + Sons)
a.i.
A
5000
1046.54
4000
1409.83
3000
2048,06
2000
1000
.i
.1
.• J.>.
1
o
5000
B
1976.06
4000
1046.54
3000
1409.81
2000
I
J J
1000
.l
,I.
o
1100
1600
2100
m/z
2465,20
.1
2465.20
125
2731.52
t ,1
2731 .52
,1, J .1.
2600
Fig. 8.4. MALD! maps of AspN protease digests of the F2-polypeptides of (A) the VRI 82-6409 and
(B) V 4 isolates of NDV. Ions at mlz values of 1046.54 and 2465.2 are the monoisotopic ions of the
internal calibrants angiotensin II and ACTH 18-39, respectively. These spectra were all collected
using delayed extraction and a digitization rate of 1 GHz. These spectra were adapted with permission
from (Lopaticki et al. 1998; Copyright, John Wiley + Sons)