Contents
Part I
Proteomics and Mass Spectrometry
Chapter 1
Strategies and Methods for Proteome Analysis ........... . 3
D. R. GOODLETT, A. TIM PERMAN, S. P. GYGI, J. WATTS,
G. CORTHALS, D. FIGEYS and R. AEBERSOLD
Chapter 2
Protein Sequencing or Genome Sequencing.
Where Does
Mass Spectrometry Fit into the Picture? ............. . ... . 15
P. ROEPSTORFF
Chapter 3
Reversible Negative Staining of Protein on Electrophoresis
Gels by Imidazole-Zinc Salts: Micropreparative Applications
to Proteome Analysis by Mass Spectrometry . . ............ 29
1. R. CASTELLANOS-SERRA, E. HARDY, W. PROENZA,
V. HUERTA, 1. J. GONZALEZ, J. P. LE CAER, R. 1. MORITZ
and R. J. SIMPSON
Chapter 4
Direct Analysis of Protein Complexes .... . ............... 53
J. R. YATES, A. J. LINK, D. SCHIELTZ, J. K. ENG
Chapter 5
De novo Sequencing of Proteins With Mass Spectrometry
Using the Differential Scanning Technique .............. . 65
M. WILM, G. NEUBAUER, 1. TAYLOR, A. SHEVCHENKO,
A. BACH!
Chapter 6
Characterization Of Gel Separated Proteins ............... 81
A.-CH. BERGMAN, M. OPPERMANN, U. OPPERMANN,
H. J(IRNVALL and T. BERGMAN
Part II
Structure Analysis
Chapter 7
Protein Structure and Dynamics by NMR in Solution ... . .. 91
B. BERSCH, M. BLACKLEDGE, B. BRUTSCHER, F. CORDIER,
J.-CH. Hus and D. MARION