62
T AKEHIKO KOJI
usually performed enzyme-immunohistochemically with antifluorochrome antibody.
As a different line of approach, the detection system using
chemiluminescence has been reported (Bijl et al. 1994). Although
they demonstrated extremely high sensitivity and easy signal
quantification, more improvement of the photon -collecting system will be needed for better morphological resolution and is
maybe beyond our present scope.
References
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction
products. J Histochem Cytochem 29:775
Bijl JJ, Rieger E, van Oostveen JW, Meijer CJLM, Oudejans CBM, Walboomers JMM (1994) Quantification ofbiotinylated RNA probes for in situ
hybridization using chemiluminescence. Histochemistry 102:77-82
Brahic M, Haase AT (1978) Detection of viral sequences oflow reiteration
frequency by in situ hybridization. Proc Natl Acad Sci USA 75:6125-6129
De Haas RR, Verwoerd NP, van der Corput MP, van Gijlswijk RP, Siitari H,
Tanke HJ (1996) The use of peroxidase-mediated deposition of biotintyramide in combination with time-resolved fluorescence imaging of
europium chelate label in immunohistochemistry and in situ hybridization. J Histochem Cytochem 44:1091-1099
Ehara H, Deguchi T, Koji T, Yang M, Ito S-I, Kawada Y (1996) Autoclave
antigen retrieval technique for immunohistochemical staining of androgen receptor in formalin-fixed paraffin sections of human prostate. Acta
Histochem Cytochem 29:311-318
Gall JG, Pardue M (1969) Formation and detection of RNA-DNA hybrid
molecules in cytological preparations. Proc Natl Acad Sci USA
63:378-383
Goto M, Koji T, Mizuno K, Tamaru M, Koikeda S, Nakane PK, Mori N, Masamune Y, Nakanishi Y (1990) Transcription switch of two phosphoglycerate kinase genes during spermatogenesis as determined with mouse
testis sections in situ. Exp Cell Res 186:273-278
HakunoN,Koji T, YanoT,KobayashiN, TsusumiO, TaketaniY, Nakane PK
(1996) Fas/ APO-l/CD95 system as a mediator of granulosa cell apoptosis
in ovarian follicle atresia. Endocrinology 137:1938-1948
Henderson GS, Conary JT, Davidson JM, Stewart SJ, House FS, McCurley TL
(1991) A reliable method for northern blot analysis using synthetic oligonucleotide probes. BioTechniques 10:190-197
John HA, Birnstiel ML, Jones KW (1969) RNA-DNA hybrids at the cytologicallevel. Nature 223:582-587
Koji T, Brenner RM (1993) Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labeled oligodeoxynucleotides. Endocrinology 132:382-392
T AKEHIKO KOJI
usually performed enzyme-immunohistochemically with antifluorochrome antibody.
As a different line of approach, the detection system using
chemiluminescence has been reported (Bijl et al. 1994). Although
they demonstrated extremely high sensitivity and easy signal
quantification, more improvement of the photon -collecting system will be needed for better morphological resolution and is
maybe beyond our present scope.
References
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction
products. J Histochem Cytochem 29:775
Bijl JJ, Rieger E, van Oostveen JW, Meijer CJLM, Oudejans CBM, Walboomers JMM (1994) Quantification ofbiotinylated RNA probes for in situ
hybridization using chemiluminescence. Histochemistry 102:77-82
Brahic M, Haase AT (1978) Detection of viral sequences oflow reiteration
frequency by in situ hybridization. Proc Natl Acad Sci USA 75:6125-6129
De Haas RR, Verwoerd NP, van der Corput MP, van Gijlswijk RP, Siitari H,
Tanke HJ (1996) The use of peroxidase-mediated deposition of biotintyramide in combination with time-resolved fluorescence imaging of
europium chelate label in immunohistochemistry and in situ hybridization. J Histochem Cytochem 44:1091-1099
Ehara H, Deguchi T, Koji T, Yang M, Ito S-I, Kawada Y (1996) Autoclave
antigen retrieval technique for immunohistochemical staining of androgen receptor in formalin-fixed paraffin sections of human prostate. Acta
Histochem Cytochem 29:311-318
Gall JG, Pardue M (1969) Formation and detection of RNA-DNA hybrid
molecules in cytological preparations. Proc Natl Acad Sci USA
63:378-383
Goto M, Koji T, Mizuno K, Tamaru M, Koikeda S, Nakane PK, Mori N, Masamune Y, Nakanishi Y (1990) Transcription switch of two phosphoglycerate kinase genes during spermatogenesis as determined with mouse
testis sections in situ. Exp Cell Res 186:273-278
HakunoN,Koji T, YanoT,KobayashiN, TsusumiO, TaketaniY, Nakane PK
(1996) Fas/ APO-l/CD95 system as a mediator of granulosa cell apoptosis
in ovarian follicle atresia. Endocrinology 137:1938-1948
Henderson GS, Conary JT, Davidson JM, Stewart SJ, House FS, McCurley TL
(1991) A reliable method for northern blot analysis using synthetic oligonucleotide probes. BioTechniques 10:190-197
John HA, Birnstiel ML, Jones KW (1969) RNA-DNA hybrids at the cytologicallevel. Nature 223:582-587
Koji T, Brenner RM (1993) Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labeled oligodeoxynucleotides. Endocrinology 132:382-392