44
YOSHINORI OTSUKI AND YUKO ITO
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Fig. 10. The statistical analysis for the labeling density of free 3' -OH DNA ends
in the uterine glandular cells in each menstrual phase, apoptotic cells and necrotic cells.
tween immunogolds and other substances; omit the staining step of lead citrate.
• Contamination showing high electron density is counted via
NIH Image program:
- Remove the contaminations from the images using a soft
prior to the process of NIH Image
program.
Applications
The ISELITEM technique is applied for confocal laser microscopy (CLM). The site of feee 3'-OH DNA ends is detected by
the reflectance from heavy metal products (anti-digoxigenin
antibody-immunogold-silver complexes), instead of DAB reac-