36
YOSHINORI OTSUKI AND YUKO ITO
Fig. 3. The grid floating on the droplet is recovered using the wire loop and is
transported to the droplet consisting of the same medium.
medium. The wire loop is used when the grid is transported between the droplets consisting of the same medium.
Note: After the incubation with the medium, carefully blot the
excessive medium both at the tip of the anti-capillary forceps and
on the face of the grid, using small pieces of filter paper.
1. Etch the ultra-thin sections with saturated sodium metaperiodate for 2 mins at RT.
2. Rinse in distilled water, for 5 mins, twice.
3. Incubate in the Equilibration buffer (Apop Tag™), for 10 to
15 secs.
4. Incubate in the mixture of Reaction buffer and TdT (Apop
Tag™) for 60 mins, at 37 °C.
5. Incubate in the Stop/wash buffer (Apop Tag™) , for 30 mins
at 37 °C.
6. Rinse in Tris-BSA buffer,S mins, three times.
YOSHINORI OTSUKI AND YUKO ITO
Fig. 3. The grid floating on the droplet is recovered using the wire loop and is
transported to the droplet consisting of the same medium.
medium. The wire loop is used when the grid is transported between the droplets consisting of the same medium.
Note: After the incubation with the medium, carefully blot the
excessive medium both at the tip of the anti-capillary forceps and
on the face of the grid, using small pieces of filter paper.
1. Etch the ultra-thin sections with saturated sodium metaperiodate for 2 mins at RT.
2. Rinse in distilled water, for 5 mins, twice.
3. Incubate in the Equilibration buffer (Apop Tag™), for 10 to
15 secs.
4. Incubate in the mixture of Reaction buffer and TdT (Apop
Tag™) for 60 mins, at 37 °C.
5. Incubate in the Stop/wash buffer (Apop Tag™) , for 30 mins
at 37 °C.
6. Rinse in Tris-BSA buffer,S mins, three times.