4 In Situ Nick End-Labeling: Electron Microscopical
33
Materials
- computer (e.g., Power Macintosh 7100/66AV, Apple Compu- Equipment
ter Inc., California, USA)
- scanner (e.g., Epson GT-6000, Epson Inc., Nagano, Japan)
- softs (e.g., Adove PhotoshopTM, Adove Systems Incorporated,
California, U.S.A.; NIH Image program, written by Wayne
Rasband at the U.S. National Institutes of Health and available
from the Internet by anonymous ftp from zippy. nimh. nih.
gOY or on floppy disk from NTIS, 5285 Port Royal RD., Springfield, VA 22161, part number PB 93-504868)
- ultramicrotome (e.g., Sorval® MT -6000, Du Pont Company,
Delaware, U.S.A.)
- transmission electron microscope (e.g., H-7100, Hitachi, Tokyo, Japan)
- incubator (e.g., ESPAC LN-122, TABAI Corp., Tokyo, Japan)
See also Fig. 2.
- nickel grids (150 meshes)
- locking forceps
- anticapillary forceps
/
wire loop
locking
Petridishe5
filter paper
forceps
dept moist with water
Fig. 2. The tools and reagents used in the ISELITEM technique.
injector with
a tilter
Tools
33
Materials
- computer (e.g., Power Macintosh 7100/66AV, Apple Compu- Equipment
ter Inc., California, USA)
- scanner (e.g., Epson GT-6000, Epson Inc., Nagano, Japan)
- softs (e.g., Adove PhotoshopTM, Adove Systems Incorporated,
California, U.S.A.; NIH Image program, written by Wayne
Rasband at the U.S. National Institutes of Health and available
from the Internet by anonymous ftp from zippy. nimh. nih.
gOY or on floppy disk from NTIS, 5285 Port Royal RD., Springfield, VA 22161, part number PB 93-504868)
- ultramicrotome (e.g., Sorval® MT -6000, Du Pont Company,
Delaware, U.S.A.)
- transmission electron microscope (e.g., H-7100, Hitachi, Tokyo, Japan)
- incubator (e.g., ESPAC LN-122, TABAI Corp., Tokyo, Japan)
See also Fig. 2.
- nickel grids (150 meshes)
- locking forceps
- anticapillary forceps
/
wire loop
locking
Petridishe5
filter paper
forceps
dept moist with water
Fig. 2. The tools and reagents used in the ISELITEM technique.
injector with
a tilter
Tools