16 Whole Mount in Situ Hybridization for mRNA Detection in Chick Embryos
233
Histological analysis of stained embryos
To process for histological analysis, the stained embryos can be
sectioned with vibratome after embedding in Gellan Gum or
agarose (Osumi et al. 1997), or with microtome after paraffinembedding according to Sasaki and Hogan (1993).
Results
Tipical results are shown in Fig. 2.
Fig. 2. Whole-mount in situ hybridization of chick embryo. (a, b) Four-day
chick embryos hybridized with probes for the T-box genes Tbx4 (a) and
Tbx5 (b) by the method described in the text. Tbx5 gene expression is observed
in the wing bud, whereas Tbx4 is expressed in the leg bud.
233
Histological analysis of stained embryos
To process for histological analysis, the stained embryos can be
sectioned with vibratome after embedding in Gellan Gum or
agarose (Osumi et al. 1997), or with microtome after paraffinembedding according to Sasaki and Hogan (1993).
Results
Tipical results are shown in Fig. 2.
Fig. 2. Whole-mount in situ hybridization of chick embryo. (a, b) Four-day
chick embryos hybridized with probes for the T-box genes Tbx4 (a) and
Tbx5 (b) by the method described in the text. Tbx5 gene expression is observed
in the wing bud, whereas Tbx4 is expressed in the leg bud.