220
AKIRA MATS UNO ET AL.
method for mRNA and encoded protein is considered to provide
an important clue for elucidating the intracellular correlation of
mRNA translation and secretion of translated protein.
The authors thank Susumu Takekoshi, Hirotoshi Utsunomiya, Yoshitaka Ohsugi and Shigeru Munakata for their dedicated assistance. This study was supported in part by the
Grant-in-aid for Scientific Research from the Ministry ofEducation, Science and Culture, the Japanese Government.
References
Egger D, Troxier M, Bienz K (1994) Light and electron microscopic in situ
hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry. J Histochem
Cytochem 42:815-822
Escaig-Haye F, Grigogiev V, Sharova I, Rudneva V, Buckrinskaya A, Fournier JG (1992) Ultrastructural localization ofHIV-1 RNA and core proteins. Simultaneous visualization using double immunogold labelling
after in situ hybridization and immunocytochemistry. J Submicrosc
Cytol Pathol 24:437-443
Gingras D, Bendayan M (1995) Colloidal gold electron microscopic in situ
hybridization: combination with immunocytochemistry for the study of
insulin and amylase secretion. Cell Vision 2:218-225
Guitteny AF, Bloch B (1989) Ultrastructural detection of the vasopressin
messenger RNA in the normal and Brattleboro rat. Histochemistry
92:277-281
Jirikowski GF, Sanna PP, Bloom FE (1990) mRNA coding for oxytocin is
present in axons of the hypothalamo-neurohypophyseal tract. Proc
Natl Acad Sci USA 87:7400-7404
Le Guellec D, Frappart L, Desprez PY (1991) Ultrastructural localization of
mRNA encoding for the EGF receptor in human breast cell cancer line
BT20 by in situ hybridization. J Histochem Cytochem 39:1-6
Le Guellec D, Frappart L, Willems R (1990) Ultrastructural localization of
fibronectin mRNA in chick embryo by in situ hybridization using 35S or
biotin labeled eDNA probes. BioI Cell 70:159-165
Le Guellec D, Trembleau A, Pechoux C, Gossard F, Morel G (1992) Ultrastructural non-radioactive in situ hybridization of GH mRNA in rat pituitary gland: preembedding vs ultrathin frozen sections vs postembedding. J Histochem Cytochem 40:979-986
Matsuno A, Ohsugi Y, Utsunomiya H, Takekoshi S, Munakata S, Nagao K,
Osamura RY, Tamura A, Nagashima T (1998) An improved ultrastructural double-staining method of rat growth hormone and its mRNA
using LR White resin: a technical note. Histochem J 30: 105-1 09
Matsuno A, Ohsugi Y, Utsunomiya H, Takekoshi S, Osamura RY, Watanabe
K, Teramoto A (1994a) Ultrastructural distribution of growth hormone,
AKIRA MATS UNO ET AL.
method for mRNA and encoded protein is considered to provide
an important clue for elucidating the intracellular correlation of
mRNA translation and secretion of translated protein.
The authors thank Susumu Takekoshi, Hirotoshi Utsunomiya, Yoshitaka Ohsugi and Shigeru Munakata for their dedicated assistance. This study was supported in part by the
Grant-in-aid for Scientific Research from the Ministry ofEducation, Science and Culture, the Japanese Government.
References
Egger D, Troxier M, Bienz K (1994) Light and electron microscopic in situ
hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry. J Histochem
Cytochem 42:815-822
Escaig-Haye F, Grigogiev V, Sharova I, Rudneva V, Buckrinskaya A, Fournier JG (1992) Ultrastructural localization ofHIV-1 RNA and core proteins. Simultaneous visualization using double immunogold labelling
after in situ hybridization and immunocytochemistry. J Submicrosc
Cytol Pathol 24:437-443
Gingras D, Bendayan M (1995) Colloidal gold electron microscopic in situ
hybridization: combination with immunocytochemistry for the study of
insulin and amylase secretion. Cell Vision 2:218-225
Guitteny AF, Bloch B (1989) Ultrastructural detection of the vasopressin
messenger RNA in the normal and Brattleboro rat. Histochemistry
92:277-281
Jirikowski GF, Sanna PP, Bloom FE (1990) mRNA coding for oxytocin is
present in axons of the hypothalamo-neurohypophyseal tract. Proc
Natl Acad Sci USA 87:7400-7404
Le Guellec D, Frappart L, Desprez PY (1991) Ultrastructural localization of
mRNA encoding for the EGF receptor in human breast cell cancer line
BT20 by in situ hybridization. J Histochem Cytochem 39:1-6
Le Guellec D, Frappart L, Willems R (1990) Ultrastructural localization of
fibronectin mRNA in chick embryo by in situ hybridization using 35S or
biotin labeled eDNA probes. BioI Cell 70:159-165
Le Guellec D, Trembleau A, Pechoux C, Gossard F, Morel G (1992) Ultrastructural non-radioactive in situ hybridization of GH mRNA in rat pituitary gland: preembedding vs ultrathin frozen sections vs postembedding. J Histochem Cytochem 40:979-986
Matsuno A, Ohsugi Y, Utsunomiya H, Takekoshi S, Munakata S, Nagao K,
Osamura RY, Tamura A, Nagashima T (1998) An improved ultrastructural double-staining method of rat growth hormone and its mRNA
using LR White resin: a technical note. Histochem J 30: 105-1 09
Matsuno A, Ohsugi Y, Utsunomiya H, Takekoshi S, Osamura RY, Watanabe
K, Teramoto A (1994a) Ultrastructural distribution of growth hormone,