15 Electron Microscopic in Situ Hybridization and its Combination
211
14. Immunohistochemical staining is carried out at room temperature for 1 hr using the appropriate antibody.
15. The grids are washed with 0.1 M PB, and the immunoreaction is visualized at room temperature for 1 hr with 20 nm
protein A colloidal gold diluted 1:40 in 0.1 M PB.
16. After being washed with 0.1 M PB and distilled water, the
grids are dried at room temperature, and observed under
an electron microscopy.
The immunohistochemical negative control experiments: substitution of normal serum for the antibody.
Results
In this section, the studies on the intracellular localization of GH
and PRL mRNA in the rat pituitary cells, to which the above deFig. 4. Photograph of pre embedding electron microscopic in situ hybridization
for rat GH mRNA. Rat GH mRNA is localized diffusely on the polysomes of the
entire rough endoplasmic reticulum. (bar=200nm) (Cited from Matsuno et al.
1994a)
211
14. Immunohistochemical staining is carried out at room temperature for 1 hr using the appropriate antibody.
15. The grids are washed with 0.1 M PB, and the immunoreaction is visualized at room temperature for 1 hr with 20 nm
protein A colloidal gold diluted 1:40 in 0.1 M PB.
16. After being washed with 0.1 M PB and distilled water, the
grids are dried at room temperature, and observed under
an electron microscopy.
The immunohistochemical negative control experiments: substitution of normal serum for the antibody.
Results
In this section, the studies on the intracellular localization of GH
and PRL mRNA in the rat pituitary cells, to which the above deFig. 4. Photograph of pre embedding electron microscopic in situ hybridization
for rat GH mRNA. Rat GH mRNA is localized diffusely on the polysomes of the
entire rough endoplasmic reticulum. (bar=200nm) (Cited from Matsuno et al.
1994a)