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HIDEFUMI YOSHIOKA AND SUMIHARE NOTI
7. Blot the bottom edge of the slide on a paper towel to remove
excess emulsion and air dry for 20 mins in a test-tube rack.
S. Place the slide in a slide box containing silica-gel particles as
desiccant.
9. Seal the box with black tape.
10. Place the box in a light-tight black plastic bag.
11. Wrap the bag in a black curtain.
12. Expose at 4° C for 14 days.
Developing 1. Transfer the slides to a slide carrier.
2. Develop the slides in Kodak D-19 developer at 20° C for
3 mins.
3. Wash in water for 1-2 mins.
4. Fix with Kodak Fixer (or Fuji fix) for 5 mins.
5. Wash the slides for 30 mins in running tap water.
Staining 1. Dip the slides in hematoxyline (Derafield) for 3 mins.
2. Wash them with running tap water for 30 mins.
3. Remove extra emulsion on the back of the slide with a razor.
4. Stain in 1 % eosin for 7 mins.
5. Dehydrate the slides in a graded series of ethanol; 50%, 70%,
90%,95%, in 100% ethanol for 15 mins three times, and then
inl00% Xylene for 15 mins three times
6. Place the slides on a paper towel, immediately add 150 III Entellan New (or Permount), cover the tissues with a coverslip.
7. Dry for at least 3 hrs, placing small weights on the coverslip to
force out any air bubbles.
Microscopy 1. Observe the slides with microscope equipped with both darkfield and bright field condensers. In the dark field, the silver
grains become visible.
HIDEFUMI YOSHIOKA AND SUMIHARE NOTI
7. Blot the bottom edge of the slide on a paper towel to remove
excess emulsion and air dry for 20 mins in a test-tube rack.
S. Place the slide in a slide box containing silica-gel particles as
desiccant.
9. Seal the box with black tape.
10. Place the box in a light-tight black plastic bag.
11. Wrap the bag in a black curtain.
12. Expose at 4° C for 14 days.
Developing 1. Transfer the slides to a slide carrier.
2. Develop the slides in Kodak D-19 developer at 20° C for
3 mins.
3. Wash in water for 1-2 mins.
4. Fix with Kodak Fixer (or Fuji fix) for 5 mins.
5. Wash the slides for 30 mins in running tap water.
Staining 1. Dip the slides in hematoxyline (Derafield) for 3 mins.
2. Wash them with running tap water for 30 mins.
3. Remove extra emulsion on the back of the slide with a razor.
4. Stain in 1 % eosin for 7 mins.
5. Dehydrate the slides in a graded series of ethanol; 50%, 70%,
90%,95%, in 100% ethanol for 15 mins three times, and then
inl00% Xylene for 15 mins three times
6. Place the slides on a paper towel, immediately add 150 III Entellan New (or Permount), cover the tissues with a coverslip.
7. Dry for at least 3 hrs, placing small weights on the coverslip to
force out any air bubbles.
Microscopy 1. Observe the slides with microscope equipped with both darkfield and bright field condensers. In the dark field, the silver
grains become visible.