116
HIDEFUMI YOSHIOKA AND SUMIHARE NOJI
Outline
A protocol of the entire procedure is illustrated in Fig. 1 (Noji
1997).
')Fixation
by perfusion
7lCover with
buffer plus probe
8)Hybridimtion
2)Postfixation
3 )Par.UIlnembedding
6)Acetylation
9)Remove Parufilm
4lCut sections
on mi ctorome :n:t
place On glass slide
5) Depararlinimtion
IO)Wash ax!
R ase treatment
~ J
4»1 ~ -I'
- j
L
.
14){)bservation
13)Oevelop
.~!'!l.!'.ls,i~~
12)Store in
II) Oip in ernul ion
~~. ~
Fig. 1. Schematic illustration of procedures for in situ hybridization (Noji
1997).