6 In Situ Hybridization for DNA: Fluorescent Probe
97
n.5 ~ ...................... ~
~ n·5 ~ .............. ~
J:"~'P ................ ====-":-.:-:-.:-:-:-:-.== 13 : : .. -: ............... -:
n. ; ~:::::::::::::::::::::.:,:::::::::::::::::::::::::::::::::::::=:~~ n·:4~::::::::::::::::::::~
n; k::::::::::~::::::·::::,::::·:.::::::::::::::::::::::::d
n: p:::::::::::,:::::::::::::::::::::::::::::' ::::::::d
n.; ~:: .. :=::.;;;::::::>::::::::::d
n~ b::::::::::::::,:::::::~:::::::::::.::::~::d
~~:. "~CC~~:~CC~
n.; ~:::::::::::,::i?:::::::::::::::::d
n·6 b· . . . . · · · · · · . . . . ·A
------18 T" -
n.6~ ................. .
-19···············
n=6~
20~
n=6~ ............ .
- - - -
21 '" ...
n=6l'~
22EE3
n·: 1 f::::::::::~~.~~:~.:J
n72~::::::,::::::::::::::::::::::d
n.~ b,,:::,,:::::::,::::::::::::::::,:::::,:~
Mean and s.d.
Fig. 9. Quantitative digital images of fluorescence intensity ratio (Kallioniemi
et al. 1994)
intensities is measured along each chromosome; the statistical
analysis of the ratios identifies regions of change in copy number
(Fig. 9). However, it does not reveal translocations, aberrations,
point mutations, and other aberrations that do not change copy
numbers. For technical reasons, ploidy changes can not be detected, and pericentromeric, heterochromatic, and telomeric regions are ignored.