6 In Situ Hybridization for DNA: Fluorescent Probe
93
Compcrative Genomic Hybridization
(Afferen1iol k:t>eIing of ~ (5OObp-2000bp)
•
• •
•
• •
•
TestDNA ~
~ Reference DNA
""r ~on Cot- J CNA
Denanxe Cl"ld preonned
• •
In-situ hybridization to normal chromosomes
I . . . . .. I
GreenPed· ··· .~ .. .. .. .... ... ~ ... ~ ... .
pier
Low IeIIeI
Deletion ~ level qlet"
anptitICaIjOn
anpliftcollon
Fig. 8. Principle of comparative genomic hybridization (Houldsworth et al.
1994)