Release and Elimination of Liver Glutamie-Oxalaeetic-Transferase
183
choledochus fistula of the dog significantly, but the amount excreted during
4 hrs is only a small fraction of the total injected. The activity of S.G.O.T.
diminishes by 12--41 ~<) during the 4 hrs following i.v. enzyme injection.
No significant arterio-hepatic venous difference in S.G.O.T. activity can be
demonstrated in dogs prior to or following G.O.T. administration; the
hepatic G.O.T. activity following G.O.T. injection never exceeds the control range. From these results it can be concluded that the liver neither
eliminates nor accumulates injected G.O.T.
A
B
Fig. 1
y
'L 50
1:'
>
B
C1J
40
Q.J
~
u
Q.J
0
t
A B
Fig. 2
Fig. 1. G.O.T.-activity in blood and liver following 10 min ligature (A) and
after recirculation of a rat liver lobe (B)
[ill]
ESSl
D
lig. liver norm. liver blood
Fig. 2. In vitro decrease of C; .0. T.-activity following 4 hrs incubation without (A)
and with (H) addition of lung slices
During 4 hrs in vitro incubation at 37°C of G.O.T. an average inactivation of 22.4 % occurs, which is not altered by the addition of liver or lung
homogenates. However, added lung slices decrease the G.O. T. activity
during 4 hrs (at 37C) from 22.4 to 47 % (Fig. 2), a highly significant
difference (t = 5.14; P < 0.001). An addition of pyridoxal-S-phosphate to
the enzyme-test (following G.O.T. injection) or to the in vitro test does not
exert any influence.
These results demonstrate, that a slight injury to the liver may bring
about a considerable increase of G.O.T. Although the problem of G.O.T.
elimination cannot vet be considered conclusively settled, it is very obvious
that the presence of relatively intact tissue (e.g. lung slices) greatly enhances enzyme inactivation. This comparatively swift process is probably
183
choledochus fistula of the dog significantly, but the amount excreted during
4 hrs is only a small fraction of the total injected. The activity of S.G.O.T.
diminishes by 12--41 ~<) during the 4 hrs following i.v. enzyme injection.
No significant arterio-hepatic venous difference in S.G.O.T. activity can be
demonstrated in dogs prior to or following G.O.T. administration; the
hepatic G.O.T. activity following G.O.T. injection never exceeds the control range. From these results it can be concluded that the liver neither
eliminates nor accumulates injected G.O.T.
A
B
Fig. 1
y
'L 50
1:'
>
B
C1J
40
Q.J
~
u
Q.J
0
t
A B
Fig. 2
Fig. 1. G.O.T.-activity in blood and liver following 10 min ligature (A) and
after recirculation of a rat liver lobe (B)
[ill]
ESSl
D
lig. liver norm. liver blood
Fig. 2. In vitro decrease of C; .0. T.-activity following 4 hrs incubation without (A)
and with (H) addition of lung slices
During 4 hrs in vitro incubation at 37°C of G.O.T. an average inactivation of 22.4 % occurs, which is not altered by the addition of liver or lung
homogenates. However, added lung slices decrease the G.O. T. activity
during 4 hrs (at 37C) from 22.4 to 47 % (Fig. 2), a highly significant
difference (t = 5.14; P < 0.001). An addition of pyridoxal-S-phosphate to
the enzyme-test (following G.O.T. injection) or to the in vitro test does not
exert any influence.
These results demonstrate, that a slight injury to the liver may bring
about a considerable increase of G.O.T. Although the problem of G.O.T.
elimination cannot vet be considered conclusively settled, it is very obvious
that the presence of relatively intact tissue (e.g. lung slices) greatly enhances enzyme inactivation. This comparatively swift process is probably