Preface
Standard molecular biology techniques are used widely in biomedical research and have had a major impact on the understanding of physiologic
processes and disease mechanisms. They also play an increasing role in molecular genetic diagnosis and new therapeutic approaches.
This laboratory manual is designed for researchers who need reliable protocols for their laboratorywork. It provides a step by step approach to standard methods of molecular biology. A wide range of topics is covered by
protocols for immediate application in the laboratory. These include purification, isolation, labeling, hybridization, sequencing and enzymatic modification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and
protein techniques.
The manual is based on experience with a practical course in molecular
biologytechniques, which was taught bythe authors. Since most methods in
molecular biology recapitulate in a test tube mechanisms that occur naturally in the cell, the first three chapters describe and illustrate basic mechanisms of molecular biology. U nderstanding more about these basic mechanisms will enable the researcher to apply techniques more specifically, based
on the knowledge of their potential and limits. W e hope that users find this
manual a helpful guide on their road to exciting insights.
Freiburg,
Spring, 1999
Friedhelm Hildebrandt
Standard molecular biology techniques are used widely in biomedical research and have had a major impact on the understanding of physiologic
processes and disease mechanisms. They also play an increasing role in molecular genetic diagnosis and new therapeutic approaches.
This laboratory manual is designed for researchers who need reliable protocols for their laboratorywork. It provides a step by step approach to standard methods of molecular biology. A wide range of topics is covered by
protocols for immediate application in the laboratory. These include purification, isolation, labeling, hybridization, sequencing and enzymatic modification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and
protein techniques.
The manual is based on experience with a practical course in molecular
biologytechniques, which was taught bythe authors. Since most methods in
molecular biology recapitulate in a test tube mechanisms that occur naturally in the cell, the first three chapters describe and illustrate basic mechanisms of molecular biology. U nderstanding more about these basic mechanisms will enable the researcher to apply techniques more specifically, based
on the knowledge of their potential and limits. W e hope that users find this
manual a helpful guide on their road to exciting insights.
Freiburg,
Spring, 1999
Friedhelm Hildebrandt