332
ECKART SCHOTT, MARTIN PAUL AND DETLEV GANTEN
ulatory elements of the gene encoding the ventricular myosin light chain 2
(MLC2v) (64, 65). Other regulatory sequences leading to specific expression
in cardiac myocytes are the sequences derived from the genes encoding
myosin heavy chain (MHC) a and ß or ANP (66, 67). Foramore detailed
review see ( 68).
In summary the availability of techniques allowing integration of larger
DNA fragmentswill offer new avenues for the application of transgenic research to medicine and biology.
References
1. Ohkubo H, Kawakami H, Kakehi Y, Takumi T, Arai H, Yokota Y, Iwai M, Tanabe Y,
Masu M, Hata J, Iwao H, Okamoto H, Yokoyama M, Nomura T, Katsuki M, Nakanishi S (1990) Generation of transgenie miee with elevated blood pressure by introduetion of the rat renin and angiotensinogen genes. Proe Natl Aead Sei USA
87:5153-5157
2. Hammer RE, Pursel VG, Reynolds RC, Rexroad CEJr, Wall RJ, Bolt DJ, Ebert KM,
Palmiter RD, Brinster RL (1985) Produetion oftransgenie rabbits, sheep and pigs by
mieroinjeetion. Nature 315:680-683
3. Steinhelper ME, Coehrane KL, Field LJ (1990) Hypotension in transgenie miee expressing atrial natriuretie faetor fusion genes. Hypertension 16:301-307
4. Field LJ, Veress AT, Steinhelper ME, Coehrane K, Sonnenberg H (1991) Kidney
funetion in ANF-transgenie miee: effeet ofblood volume expansion. Am J Physiol
260:R1-R5
5. Katsuki M, Sato M, Kimura M, Yokoyama M, Kobayashi K, Nomura T (1988) Conversion of normal behavior to shiverer by myelin basie protein antisense eDNA in
transgenie miee. Seienee 241:593-595
6. Jat PS, Noble MD, Ataliotis P, Tanaka Y, Yannoutsos N, Larsen L, Kioussis D (1991)
Direet derivation of eonditionally immortal eelllines from a H-2Kb-tsA58 transgenie mouse. Proe Natl Aead Sei USA; 88 (12):5096 -100
7. Hayes C, Kelly D, Murayama S, Komiyama A, Suzuki K, Popko B (1992) Expression
of the neu oneogene under the transeriptional eontrol of the myelin basie protein
gene in transgenie miee: generation of transformed glial eells. Neurosei Res
31(1):175-87
8. Babinet C, Morello D, Renard J (1989) Transgenie miee. Genome 31:938-949
9. Gordon JW, Ruddle FH (1983) Genetransfer into mouse embryos: produetion of
transgenie miee by pronuclear injeetion. Methods Enzymol101:411-433
10. Evans G, Armstrong D (1984) Reduetion in fertilization rate in-vitro of ooeytes from
immature rats indueed to superovulate. J Reprod Fertil 70:131-135
11. Robertson E ( 1991) U sing embryonie stem eells to introduee mutations into mouse
germ line. Biol Reprod 44:238-245
12. Williams DA (1990) Embryonie stem eells targets for gene transfer: a new approaeh
to moleeular manipulation of the murine hematopoietie system. Bone marrow
transplantation 5:141-144
13. Hogan B, Costantini F, Laey E (1986) Manipulating the mouse embryo. Cold Spring
Harbor Laboratory, Cold Spring Harbor, New York 11724
ECKART SCHOTT, MARTIN PAUL AND DETLEV GANTEN
ulatory elements of the gene encoding the ventricular myosin light chain 2
(MLC2v) (64, 65). Other regulatory sequences leading to specific expression
in cardiac myocytes are the sequences derived from the genes encoding
myosin heavy chain (MHC) a and ß or ANP (66, 67). Foramore detailed
review see ( 68).
In summary the availability of techniques allowing integration of larger
DNA fragmentswill offer new avenues for the application of transgenic research to medicine and biology.
References
1. Ohkubo H, Kawakami H, Kakehi Y, Takumi T, Arai H, Yokota Y, Iwai M, Tanabe Y,
Masu M, Hata J, Iwao H, Okamoto H, Yokoyama M, Nomura T, Katsuki M, Nakanishi S (1990) Generation of transgenie miee with elevated blood pressure by introduetion of the rat renin and angiotensinogen genes. Proe Natl Aead Sei USA
87:5153-5157
2. Hammer RE, Pursel VG, Reynolds RC, Rexroad CEJr, Wall RJ, Bolt DJ, Ebert KM,
Palmiter RD, Brinster RL (1985) Produetion oftransgenie rabbits, sheep and pigs by
mieroinjeetion. Nature 315:680-683
3. Steinhelper ME, Coehrane KL, Field LJ (1990) Hypotension in transgenie miee expressing atrial natriuretie faetor fusion genes. Hypertension 16:301-307
4. Field LJ, Veress AT, Steinhelper ME, Coehrane K, Sonnenberg H (1991) Kidney
funetion in ANF-transgenie miee: effeet ofblood volume expansion. Am J Physiol
260:R1-R5
5. Katsuki M, Sato M, Kimura M, Yokoyama M, Kobayashi K, Nomura T (1988) Conversion of normal behavior to shiverer by myelin basie protein antisense eDNA in
transgenie miee. Seienee 241:593-595
6. Jat PS, Noble MD, Ataliotis P, Tanaka Y, Yannoutsos N, Larsen L, Kioussis D (1991)
Direet derivation of eonditionally immortal eelllines from a H-2Kb-tsA58 transgenie mouse. Proe Natl Aead Sei USA; 88 (12):5096 -100
7. Hayes C, Kelly D, Murayama S, Komiyama A, Suzuki K, Popko B (1992) Expression
of the neu oneogene under the transeriptional eontrol of the myelin basie protein
gene in transgenie miee: generation of transformed glial eells. Neurosei Res
31(1):175-87
8. Babinet C, Morello D, Renard J (1989) Transgenie miee. Genome 31:938-949
9. Gordon JW, Ruddle FH (1983) Genetransfer into mouse embryos: produetion of
transgenie miee by pronuclear injeetion. Methods Enzymol101:411-433
10. Evans G, Armstrong D (1984) Reduetion in fertilization rate in-vitro of ooeytes from
immature rats indueed to superovulate. J Reprod Fertil 70:131-135
11. Robertson E ( 1991) U sing embryonie stem eells to introduee mutations into mouse
germ line. Biol Reprod 44:238-245
12. Williams DA (1990) Embryonie stem eells targets for gene transfer: a new approaeh
to moleeular manipulation of the murine hematopoietie system. Bone marrow
transplantation 5:141-144
13. Hogan B, Costantini F, Laey E (1986) Manipulating the mouse embryo. Cold Spring
Harbor Laboratory, Cold Spring Harbor, New York 11724