15 Cloning Vectors
Common Vectors and Host Cells for Molecular Cloning
Vector
Host Cell
Growth Medium Supplements
pBR322
HB101 (Boyer 1969)
LB
antibiotics
DH1 (Hanahan 1983)
pUC
JM103 (Messing 1981)
LB
ampicillin
XLl-blue™
pSP6/T7
JM103
LB
ampicillin
XLl-blue™
EMBL3
LE392
LB
MgS04
Cosmids
BHB2688 (Hohn 1979)
LB
BHB2690
M13
JM103 ; JM107
minimal medium
lcgtlO
C600 (Appleyard 1954)
LB
MgS04
lcgtll
Y1090
LB
ampicillin; MgS04
remain the same, but appropriate medium, antibiotics or different reagents
for selective growth must be used.
Streak E. coli HBlOl on a stock plate to obtain single colonies. This stock
plate can be stored at 4°C for about 1 month. After that you have to replace
the stock plate.
Materials
Plates for bacterial growth
• Bacto-tryptone
• Bacto-yeast extract
• NaCl
• Agar
• 1M NaOH
• Ampicillin, 50 mg/ml, sterile-filtered
Reagents
Solutions
235