Lambda DNA
genomic DNA
EcoRI
EcoRI
EcoRI
restriction
ligation
recombinant Iambda phage concatamere
+Cj?
hasteeil
( packaging
extract)
transfection
15 Cloning Vectors
231
Fig. 3. Cloning of a DNA fragment into Lambda phage and subsequent Iransfeetion ofhost
cells after packaging the recombinant DNA into phage particles