ddATP
-
-
T
22 bp
21 bp
20 bp
19 bp
16 bp
17 bp
16 bp
15 bp
14 bp
13 bp
12 bp
11 bp
10 bp
9 bp
13 Sequencing
189
Fig. 4. The sequencing ladder resulting from the ddA TP reaction of Figure 3 after separation
on a polyacrylamide gel.
ddATP
ddTTP
ddC P
ddGTP
-
22 bp
-
21 bp
20 bp
-
19 bp
-
16 bp
17 bp
16 bp
-
15 bp
-
14 bp
13 bp
-
12 bp
-
11 bp
-
10 bp
9 bp
T
A G
c
Fig. 5. The sequencing ladders resulting from all 4 ddNTP reactions of Figure 3 giving the
complete sequence.