X
Contents
Subprotocol 5: Denaturation of the DNA on the Slides ........... 176
Subprotocol 6: Detection .................................. 178
Subprotocol 7: Chromosomal in situ Suppression (CISS)
Hybridization ............................... 179
Subprotocol 8: Signal Amplification .......................... 181
Subprotocol 9: Multicolor Fluorescence in situ Hybridization ...... 182
Chapter 13
Sequencing
KLAUS DEICHMANN •..••••.•••••••••..•..•••••.•.•...••.. 186
Subprotocol1: Standard protocol for T7 DNA polymerase
(Sequenase™) .............................. 194
Subprotocol 2: Standard Protocol for Sequencing with Taq
Polymerase ................................. 197
Subprotocol 3: Denaturing Polyacrylamide Sequencing Gels ....... 200
Chapter 14
Polymerase Chain Reaction
FRIEDHELM HILDEBRANDT AND IVA SINGH-SA WHNEY •.•.•.•.•• 207
Chapter 15
Cloning V ectors
ANNETTE DEICHMANN AND KLAUS DEICHMANN .•.••.••.•..••. 226
Subprotocol 1: Transformation of Bacteria with Recombinant
Plasmid Using the Ca++ -Precipitation Method
(Mandel1970) .............................. 234
Subprotocol 2: Transformation of Bacteria with Recombinant
Plasmid by Electroporation (Dower 1988) ......... 238
Subprotocol 3: Transformation of Bacteria with Recombinant
Bacteriophage DNA .......................... 241
Subprotocol 4: "Mini-Prep" Method for Plasmid Isolation ........ 245
Subprotocol 5: Chromatographie Method for Plasmid Preparation .. 247
Subprotocol 6: Large-Scale Preparation of Bacteriophage DNA
(Replicative Form (RF)) ....................... 249