On June 23 and 25 (at the third and fifth days of experiment, correspondingly)
we conducted the first studies of K. nova spores in artificially created conditions. It
appeared that in the fresh water there was considerably greater number of anomalous (deformed – with changed form of valves, with shift of polar capsules from
their axes to chaotic placing) spores – from 12 to 48% in the refrigerator and from
9 to 17% under the street temperature (Table 29.1).
The anomalous spores number in the sea water in refrigerator made 4–6%, while
in crystallizers behind the window their share made 4–10%. In the water media,
contained in refrigerator (with water temperature + 16
C) microflora practically
was not developed, while with water temperature + 18
C in crystallizers behind the
window there was great number of bacteria in water (Figs. 29.1, 29.2, 29.3 and
29.4).
On June 30, at the tenth day of experiment we observed in the fresh water in
refrigerator already 67% anomalous spores, while in the sea water in refrigerator
there were only 2% of such spores. In the crystallizers under natural weather
conditions we observed 11% of anomalous spores in the fresh water and 20% in
the brine.
On July 4, at the 14-th day of experiment we noted in the fresh water in
refrigerator 38% ugly spores and in the brine again only 2%. In the crystallizers
under natural weather conditions we observed in the muscles of gobies from the
fresh water 13% of anomalous spores, in the sea water spores were not found and
only mass of Protozoa and bacteria was observed.
On July 10, at the 20-th day of experiment we fixed in the refrigerators in fresh
water 64% of anomalous spores, in the sea water still low magnitude (3%). Great
number of darkened parasite spores with dark polar capsules began to be met. In the
crystallizers under the street temperature muscles in the fresh and sea water
represented slimy mass in which spores were not revealed.
On July 18, at the 28-th day of experiment in the refrigerator in fresh water we
found 83% ugly spores and in the sea water only 4%. Most of anomalous spores
were of dark colour. Placing such spores into 8% alkaline solution KOH has shown
that in such spores extrusion of the polar filament does not take place for many
hours.
On July 24, at 34-th day of experiment K. nova spores were found only in the
fresh water in refrigerator. The share of anomalous spores reached 87%.
Thus it has been revealed that already at the third day of experiment a bit less
than a half of spores in cysts which were put into fresh water had anomalous
construction. To the 34-th day a number of such ugly forms grew to 87%. The
most comfortable conditions were created in cysts placed to the sea water in
refrigerator, where to the contrary number of normal spores made from 94 to
98% along the period of experimental studies, which lasted for more than a
month. As for cysts in muscles, here their location in the host tissues in a definite
biochemical medium played great role in preservation of spores. In such case a
number of anomalous spores in the fresh and sea water under the street temperature
was correlative and quite not high – in the fresh water it varied from 9 to 17%, in the
sea water – from 4 to 20%.
29 Effect of Water Different Salinity on the Morphology of Kudoa nova. . .
497
we conducted the first studies of K. nova spores in artificially created conditions. It
appeared that in the fresh water there was considerably greater number of anomalous (deformed – with changed form of valves, with shift of polar capsules from
their axes to chaotic placing) spores – from 12 to 48% in the refrigerator and from
9 to 17% under the street temperature (Table 29.1).
The anomalous spores number in the sea water in refrigerator made 4–6%, while
in crystallizers behind the window their share made 4–10%. In the water media,
contained in refrigerator (with water temperature + 16
C) microflora practically
was not developed, while with water temperature + 18
C in crystallizers behind the
window there was great number of bacteria in water (Figs. 29.1, 29.2, 29.3 and
29.4).
On June 30, at the tenth day of experiment we observed in the fresh water in
refrigerator already 67% anomalous spores, while in the sea water in refrigerator
there were only 2% of such spores. In the crystallizers under natural weather
conditions we observed 11% of anomalous spores in the fresh water and 20% in
the brine.
On July 4, at the 14-th day of experiment we noted in the fresh water in
refrigerator 38% ugly spores and in the brine again only 2%. In the crystallizers
under natural weather conditions we observed in the muscles of gobies from the
fresh water 13% of anomalous spores, in the sea water spores were not found and
only mass of Protozoa and bacteria was observed.
On July 10, at the 20-th day of experiment we fixed in the refrigerators in fresh
water 64% of anomalous spores, in the sea water still low magnitude (3%). Great
number of darkened parasite spores with dark polar capsules began to be met. In the
crystallizers under the street temperature muscles in the fresh and sea water
represented slimy mass in which spores were not revealed.
On July 18, at the 28-th day of experiment in the refrigerator in fresh water we
found 83% ugly spores and in the sea water only 4%. Most of anomalous spores
were of dark colour. Placing such spores into 8% alkaline solution KOH has shown
that in such spores extrusion of the polar filament does not take place for many
hours.
On July 24, at 34-th day of experiment K. nova spores were found only in the
fresh water in refrigerator. The share of anomalous spores reached 87%.
Thus it has been revealed that already at the third day of experiment a bit less
than a half of spores in cysts which were put into fresh water had anomalous
construction. To the 34-th day a number of such ugly forms grew to 87%. The
most comfortable conditions were created in cysts placed to the sea water in
refrigerator, where to the contrary number of normal spores made from 94 to
98% along the period of experimental studies, which lasted for more than a
month. As for cysts in muscles, here their location in the host tissues in a definite
biochemical medium played great role in preservation of spores. In such case a
number of anomalous spores in the fresh and sea water under the street temperature
was correlative and quite not high – in the fresh water it varied from 9 to 17%, in the
sea water – from 4 to 20%.
29 Effect of Water Different Salinity on the Morphology of Kudoa nova. . .
497