Dissolved Oxygen
79
Apparatus and Supplies
1. Manganous sulfate reagent (400 g MnS0 4 ·2H 2 0 or 364 g MnS04 • H 20 dissolved in 1 liter
of distilled water).
2. Alkaline-iodide-azide reagent (500g NaOH or 700g KOH and 135g NaI or 150g KI
dissolved in one liter of distilled water; 10 g NaN 3 (Caution: extremely poisonous) in 40 ml
distilled water, added to 960ml of alkaline-iodide reagent).
3. Standardized sodium thiosulfate solution (either (a) or (b)):
a. 0.005M, prepared from O.lM stock solution (kept refrigerated). Remove O.1M Na2S20 3
and allow to come to room temperature; dilute 50ml to 1 liter with distilled water.
b. 0.025M (6.205 g NazS203· 5H 2 0 dissolved in 1 liter offreshly boiled and cooled distilled
water). 1.00 ml of 0.025N Na2S203 solution = 0.200 mg dissolved oxygen.
Standardization:
i. Dissolve 2 g Kl in 100 to 150-ml distilled water.
ii. Add 10ml of 1:9 H 2S04 .
iii. Add exactly 20.00ml 0.0021 M KH(103h. Dissolve 812.4mg KH (I03h in 1 liter of
distilled water.
iv. Dilute to 200ml with distilled water.
v. Titrate with the thiosulfate solution using procedures identical to those for dissolved
oxygen.
vi. Calculate molarity of the thiosulfate solution
(Volt) (M 1 )=(VoI 2 )(M2) [cf., Appendix 1]
4. Stabilized starch solution [an excellent stabilized starch solution which will not deteriorate
for years, is given by Van Landingham (1960)].
5. Concentrated sulfuric acid.
6. Automatic dispensing pipets, 2 ml, adjustable.
7. Burettes (50 ml) and volumetric pipettes (100 ml, 20 ml).
8. Erlenmeyer flasks or porcelain casserole dishes (250 to 300 ml).
9. BOD oxygen bottles with tapered stoppers.
10. Underwater thermometer.
11. Mercurial barometer (optional).
12. Planimeter or computerized digitizer.
References
American Public Health Association et al. 1989. Standard Methods for the Examination of
Water, Sewage, and Wastewater, 17th Ed. American Public Health Association, New York.
1550pp.
Benson, B.B. and D. Krause, Jr. 1980. The concentration and isotopic fractionation of gases
dissolved in fresh water in equilibrium with the atmosphere. 1. Oxygen. Limnol. Oceanogr.
25: 662-671.
Buswell, A.M. and W.U. Gallaher. 1923. Determination of dissolved oxygen in the presence of
iron salts. Ind. Eng. Chem. 15: 1186-1188.
Carlton, R.G. and R.G. Wetzel. 1987. Distribution and fates of oxygen in periphyton
communities. Can. J. Bot. 65: 1031-1037.
Carlton, R.G. and R.G. Wetzel. 1988. Phosphorus flux from lake sediments: Effect of epipelic
algal oxygen production. Limnol. Oceanogr. 33: 562-570.
Ellis, 1. and S. Kanamori. 1973. An evaluation of the Miller method for dissolved oxygen analysis.
Limnol. Oceanogr. 18: 1002-1005.
Gnaiger, E. and H. Forstner (ed). 1983. Polarographic Oxygen Sensors: Aquatic and
Physiological Applications. Springer-Verlag, New York. 370pp.
Hutchinson, G.E. 1957. A Treatise on Limnology. I. Geography, Physics, and Chemistry. Wiley,
New York. 1015pp.
79
Apparatus and Supplies
1. Manganous sulfate reagent (400 g MnS0 4 ·2H 2 0 or 364 g MnS04 • H 20 dissolved in 1 liter
of distilled water).
2. Alkaline-iodide-azide reagent (500g NaOH or 700g KOH and 135g NaI or 150g KI
dissolved in one liter of distilled water; 10 g NaN 3 (Caution: extremely poisonous) in 40 ml
distilled water, added to 960ml of alkaline-iodide reagent).
3. Standardized sodium thiosulfate solution (either (a) or (b)):
a. 0.005M, prepared from O.lM stock solution (kept refrigerated). Remove O.1M Na2S20 3
and allow to come to room temperature; dilute 50ml to 1 liter with distilled water.
b. 0.025M (6.205 g NazS203· 5H 2 0 dissolved in 1 liter offreshly boiled and cooled distilled
water). 1.00 ml of 0.025N Na2S203 solution = 0.200 mg dissolved oxygen.
Standardization:
i. Dissolve 2 g Kl in 100 to 150-ml distilled water.
ii. Add 10ml of 1:9 H 2S04 .
iii. Add exactly 20.00ml 0.0021 M KH(103h. Dissolve 812.4mg KH (I03h in 1 liter of
distilled water.
iv. Dilute to 200ml with distilled water.
v. Titrate with the thiosulfate solution using procedures identical to those for dissolved
oxygen.
vi. Calculate molarity of the thiosulfate solution
(Volt) (M 1 )=(VoI 2 )(M2) [cf., Appendix 1]
4. Stabilized starch solution [an excellent stabilized starch solution which will not deteriorate
for years, is given by Van Landingham (1960)].
5. Concentrated sulfuric acid.
6. Automatic dispensing pipets, 2 ml, adjustable.
7. Burettes (50 ml) and volumetric pipettes (100 ml, 20 ml).
8. Erlenmeyer flasks or porcelain casserole dishes (250 to 300 ml).
9. BOD oxygen bottles with tapered stoppers.
10. Underwater thermometer.
11. Mercurial barometer (optional).
12. Planimeter or computerized digitizer.
References
American Public Health Association et al. 1989. Standard Methods for the Examination of
Water, Sewage, and Wastewater, 17th Ed. American Public Health Association, New York.
1550pp.
Benson, B.B. and D. Krause, Jr. 1980. The concentration and isotopic fractionation of gases
dissolved in fresh water in equilibrium with the atmosphere. 1. Oxygen. Limnol. Oceanogr.
25: 662-671.
Buswell, A.M. and W.U. Gallaher. 1923. Determination of dissolved oxygen in the presence of
iron salts. Ind. Eng. Chem. 15: 1186-1188.
Carlton, R.G. and R.G. Wetzel. 1987. Distribution and fates of oxygen in periphyton
communities. Can. J. Bot. 65: 1031-1037.
Carlton, R.G. and R.G. Wetzel. 1988. Phosphorus flux from lake sediments: Effect of epipelic
algal oxygen production. Limnol. Oceanogr. 33: 562-570.
Ellis, 1. and S. Kanamori. 1973. An evaluation of the Miller method for dissolved oxygen analysis.
Limnol. Oceanogr. 18: 1002-1005.
Gnaiger, E. and H. Forstner (ed). 1983. Polarographic Oxygen Sensors: Aquatic and
Physiological Applications. Springer-Verlag, New York. 370pp.
Hutchinson, G.E. 1957. A Treatise on Limnology. I. Geography, Physics, and Chemistry. Wiley,
New York. 1015pp.