Decomposition: Relative Bacterial Heterotrophic Activity
279
nonlabeled substrate can be (i) mixed with the labeled material in such a way that added,ul
amounts (Procedures, Section 3) contain final substrate concentrations of 10 to 500,ug/1
when diluted with the water sample, or (ii) a constant amount oflabeled substrate is added
to each flask and supplemented with increasing concentrations of nonlabeled substrate.
When the latter method is used, several stock solutions of increasing concentrations
should be prepared so that a constant volume of substrate is added to each flask.
11. Van Dorn water sampler.
12. Electrical underwater thermometer.
References
Burnison, B.K. and R.Y. Morita. 1973. Competitive inhibition for amino acid uptake by the
indigenous microflora of Upper Klamath Lake. Appl. Microbiol. 25: 103-106.
Caldwell, D.E. 1977. The planktonic microflora of lakes. CRC Critical Rev. Microbiol. 5:
305-370.
Cunningham, H.W. and RG. Wetzel, 1989. Kinetic analysis of protein degradation by a
freshwater wetland sediment community. Appl. Environ. Microbiol. 55: 1963-1967.
Harrison, MJ., RT. Wright, and RY. Morita. 1971. Method for measuring mineralization in
lake sediments. Appl. Microbiol. 21: 698-702.
Hobbie, J.E. and C.E. Crawford. 1969a. Respiration corrections for bacterial uptake of dissolved
organic compounds in natural waters. Limnol. Oceanogr. 14: 528-532.
Hobbie, IE. and C.c. Crawford. 1969b. Bacterial uptake of organic substrate: New methods of
study and application to eutrophication. Verh. Int. Ver. Limnol. 17: 725-730.
Hobbie, J.E. and RT. Wright. 1965. Bioassay with bacterial uptake kinetics: Glucose in
freshwater. Limnol. Oceanogr. 10: 471-474.
Meyer-Reil, L.A. 1978. Uptake of glucose by bacteria in the sediment. M.ar. BioI. 44: 293-298.
Parsons, T.R and J.D.H. Strickland. 1962. On the production of particulate organic carbon by
heterotrophic processes in sea water. Deep-Sea Res. 8: 211-222.
Sorokin, Y.I. and H. Kadota (eds). 1972. Techniques for the Assessment of Microbial Production
and Decomposition in Fresh Waters. IBP Handbook No. 23, Blackwell, Oxford. 112pp.
Wetzel, R.G. 1983. Limnology. 2nd Ed. Saunders Coli., Philadelphia. 860pp.
Wright, RT. 1973. Some difficulties in using 14C-organic solutes to measure heterotrophic
bacterial activity. pp. 199-217. In: L.H. Stevenson and R.R. Colwell, Editors. Estuarine
Microbial Ecology. Univ. South Carolina Press, Columbia.
Wright, RT. and IE. Hobbie. 1965. The uptake of organic solutes in lake water. Limnol.
Oceanogr. 10: 22-28.
Wright, R.T. and IE. Hobbie. 1966. The use of glucose and acetate by bacteria and algae in
aquatic ecosystems. Ecology 47: 447-464.
279
nonlabeled substrate can be (i) mixed with the labeled material in such a way that added,ul
amounts (Procedures, Section 3) contain final substrate concentrations of 10 to 500,ug/1
when diluted with the water sample, or (ii) a constant amount oflabeled substrate is added
to each flask and supplemented with increasing concentrations of nonlabeled substrate.
When the latter method is used, several stock solutions of increasing concentrations
should be prepared so that a constant volume of substrate is added to each flask.
11. Van Dorn water sampler.
12. Electrical underwater thermometer.
References
Burnison, B.K. and R.Y. Morita. 1973. Competitive inhibition for amino acid uptake by the
indigenous microflora of Upper Klamath Lake. Appl. Microbiol. 25: 103-106.
Caldwell, D.E. 1977. The planktonic microflora of lakes. CRC Critical Rev. Microbiol. 5:
305-370.
Cunningham, H.W. and RG. Wetzel, 1989. Kinetic analysis of protein degradation by a
freshwater wetland sediment community. Appl. Environ. Microbiol. 55: 1963-1967.
Harrison, MJ., RT. Wright, and RY. Morita. 1971. Method for measuring mineralization in
lake sediments. Appl. Microbiol. 21: 698-702.
Hobbie, J.E. and C.E. Crawford. 1969a. Respiration corrections for bacterial uptake of dissolved
organic compounds in natural waters. Limnol. Oceanogr. 14: 528-532.
Hobbie, IE. and C.c. Crawford. 1969b. Bacterial uptake of organic substrate: New methods of
study and application to eutrophication. Verh. Int. Ver. Limnol. 17: 725-730.
Hobbie, J.E. and RT. Wright. 1965. Bioassay with bacterial uptake kinetics: Glucose in
freshwater. Limnol. Oceanogr. 10: 471-474.
Meyer-Reil, L.A. 1978. Uptake of glucose by bacteria in the sediment. M.ar. BioI. 44: 293-298.
Parsons, T.R and J.D.H. Strickland. 1962. On the production of particulate organic carbon by
heterotrophic processes in sea water. Deep-Sea Res. 8: 211-222.
Sorokin, Y.I. and H. Kadota (eds). 1972. Techniques for the Assessment of Microbial Production
and Decomposition in Fresh Waters. IBP Handbook No. 23, Blackwell, Oxford. 112pp.
Wetzel, R.G. 1983. Limnology. 2nd Ed. Saunders Coli., Philadelphia. 860pp.
Wright, RT. 1973. Some difficulties in using 14C-organic solutes to measure heterotrophic
bacterial activity. pp. 199-217. In: L.H. Stevenson and R.R. Colwell, Editors. Estuarine
Microbial Ecology. Univ. South Carolina Press, Columbia.
Wright, RT. and IE. Hobbie. 1965. The uptake of organic solutes in lake water. Limnol.
Oceanogr. 10: 22-28.
Wright, R.T. and IE. Hobbie. 1966. The use of glucose and acetate by bacteria and algae in
aquatic ecosystems. Ecology 47: 447-464.