Support block
Burner
Organic Matter
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___
~=:::::j::::====' :."'< ,"- ~
gas/oxygen
131
Figure 9.1. Sealing of ampoules under a continuous sparging of head space with a stream of
oxygen. A support block of aluminium with a set screw to hold the cannula is affixed to the upper
side of the pliers. The cannula is joined with Teflon or other heat-resistant tubing to the tubing
that delivers the sparging gas. [From McDowell et al. (1987).J
9. Place the ampoule firmly into the silicone tubing with the cannula sticking through
the silicone tubing above it (see Fig. 9.2). Start the flow ofN 2 gas (200 ml/min) to the
infrared CO 2 spectrometer and maintain this flow until the recorder reads
zero.
Crush the end of the ampoule within the tubing with a pair of pliers. Quickly
push the cannula down into the ampoule until the tip is well under the liquid.
Continue passing N 2 until the recorder returns to zero. Remove the ampoule,
1. Ascante
5. Ampoule
2. PrecIsion Flowmeter
6. CaCI 2
3. Cannula
7. IR Analyzer
4. Silicone Tubing
8. Amplifier and Recorder
Figure 9.2. Closed system to sparge CO 2 of dissolved organic matter to an infrared spectrophotometer for analysis.