422
Fig. 18.5. Chronopotentiograms
obtained using calf thymus
ssDNA modified screen printed
electrode with: (a) blank solution, (b), (e) and (d) river water
(100 times concentrated). Calf
thymus ssDNA immobilization:
20 mg t l calf thymus ssDNA
for 2 min at +0.5 V vs. SCE. PSA
transduction: in 0.2 M acetate
buffer pH 5.0 with a stripping
constant current of +6 flA and
an initial potential of +0.5 V
References
~
~
~
.....
~
M.Mascini
1.60 " - - - - - - - - - - - - - - - - - - - - - ,
0.95
1.00
a
1.05
E(V)
a Peakarea:89±13ms
b Peak area: 94 ± 7 ms
c Peak area: 61 ± 8 ms
d Peak area: 48 ± 9 ms
1.10
1.15
Del Carlo M, Lionti I, Taccini M, Cagnini A, Mascini M (1997) Disposable screen-printed electrodes for
the immunochemical detection of polychlorinated biphenyls. Anal Chim Acta 342:189
Gopalakrishnan S, Byrd S, Stone MP, Harris TM (1989) Carcinogen-nucleic acid interactions: Equilibrium binding studies of aflatoxin BI with the oligodeoxynucleotide d(ATGCATh and with plasmid
pBR322 support intercalative association with the B-DNA helix. Biochemistry 28:726
Marrazza G, Chianella I, Mascini M (1999a) Disposable DNA electrochemical sensor for hybridization
detection. Biosensors and Bioelectronics 14:43
Marrazza G, Chianella I, Mascini M (1999b) Disposable DNA electrochemical biosensors for environmental monitoring. Anal Chim Acta 387:297
Mecklenburg M, Grauers A, Rees Jonsson B, Weber A, Danielsson B (1997) A strategy for the broad range
detection of compounds with affinity for nucleic acids. Anal Chim Acta 347:79
Jelen F, Fojta M, Palecek E (1997a) Voltammetry of native double-stranded, denatured and degraded DNAs.
J Electroanal Chern 427:49
Jelen F, Tomoschik M, Palecek E (1997b) Adsorptive stripping square-wave voltammetry of DNA.
J Electroanal Chern 423:141
Wang J, Rivas G, Cai X, Shiraishi H, Farias PAM, Dontha N, Luo D (1996a) Accumulation and trace measurements of phenothiazine drugs at DNA-modified electrodes. Anal Chim Acta 332:139
Wang J, Chicharro M, Rivas G, Cai X, Dontha N, Farias PAM, Shiraishi H (1996b) DNA biosensor for the
detection of hydrazines. Anal Chern 68(13):2251
Wang J, Rivas G, Luo D, Cai X, Valera FS, Dontha N (1996c) DNA-modified electrode for the detection of
aromatic amines. Anal Chern 68(24):4365
Wang J, Cai X, Rivas G, Shiraishi H, Dontha N (1997a) Nucleic-acid immobilization, recognition and
detection at chronopotentiometric DNA chips. Biosensors and Bioelectronics 12(7):587
Wang J, Rivas G, Palecek E, Nielsen P, Shiraishi H, Dontha N, Luo D, Parrado C, Chicharro M, Farias PAM,
Valera FS, Grant DH, Ozsoz M (1997b) DNA electrochemical biosensors for environmental monitoring. A review. Anal Chim Acta 347:1
Fig. 18.5. Chronopotentiograms
obtained using calf thymus
ssDNA modified screen printed
electrode with: (a) blank solution, (b), (e) and (d) river water
(100 times concentrated). Calf
thymus ssDNA immobilization:
20 mg t l calf thymus ssDNA
for 2 min at +0.5 V vs. SCE. PSA
transduction: in 0.2 M acetate
buffer pH 5.0 with a stripping
constant current of +6 flA and
an initial potential of +0.5 V
References
~
~
~
.....
~
M.Mascini
1.60 " - - - - - - - - - - - - - - - - - - - - - ,
0.95
1.00
a
1.05
E(V)
a Peakarea:89±13ms
b Peak area: 94 ± 7 ms
c Peak area: 61 ± 8 ms
d Peak area: 48 ± 9 ms
1.10
1.15
Del Carlo M, Lionti I, Taccini M, Cagnini A, Mascini M (1997) Disposable screen-printed electrodes for
the immunochemical detection of polychlorinated biphenyls. Anal Chim Acta 342:189
Gopalakrishnan S, Byrd S, Stone MP, Harris TM (1989) Carcinogen-nucleic acid interactions: Equilibrium binding studies of aflatoxin BI with the oligodeoxynucleotide d(ATGCATh and with plasmid
pBR322 support intercalative association with the B-DNA helix. Biochemistry 28:726
Marrazza G, Chianella I, Mascini M (1999a) Disposable DNA electrochemical sensor for hybridization
detection. Biosensors and Bioelectronics 14:43
Marrazza G, Chianella I, Mascini M (1999b) Disposable DNA electrochemical biosensors for environmental monitoring. Anal Chim Acta 387:297
Mecklenburg M, Grauers A, Rees Jonsson B, Weber A, Danielsson B (1997) A strategy for the broad range
detection of compounds with affinity for nucleic acids. Anal Chim Acta 347:79
Jelen F, Fojta M, Palecek E (1997a) Voltammetry of native double-stranded, denatured and degraded DNAs.
J Electroanal Chern 427:49
Jelen F, Tomoschik M, Palecek E (1997b) Adsorptive stripping square-wave voltammetry of DNA.
J Electroanal Chern 423:141
Wang J, Rivas G, Cai X, Shiraishi H, Farias PAM, Dontha N, Luo D (1996a) Accumulation and trace measurements of phenothiazine drugs at DNA-modified electrodes. Anal Chim Acta 332:139
Wang J, Chicharro M, Rivas G, Cai X, Dontha N, Farias PAM, Shiraishi H (1996b) DNA biosensor for the
detection of hydrazines. Anal Chern 68(13):2251
Wang J, Rivas G, Luo D, Cai X, Valera FS, Dontha N (1996c) DNA-modified electrode for the detection of
aromatic amines. Anal Chern 68(24):4365
Wang J, Cai X, Rivas G, Shiraishi H, Dontha N (1997a) Nucleic-acid immobilization, recognition and
detection at chronopotentiometric DNA chips. Biosensors and Bioelectronics 12(7):587
Wang J, Rivas G, Palecek E, Nielsen P, Shiraishi H, Dontha N, Luo D, Parrado C, Chicharro M, Farias PAM,
Valera FS, Grant DH, Ozsoz M (1997b) DNA electrochemical biosensors for environmental monitoring. A review. Anal Chim Acta 347:1