490
3
Chemical Glycosylation Reactions
⊡ Scheme 25
fully carried out, partly by following known procedures [155,156]. Also an important Galβ
(1–4)GlcNAcβ(1–3)-L-Fuc moiety was prepared, which is part of O-linked chains of human
clotting factor IX [157].
More demanding is direct glycosylation of peptides, which was so far not very successful
because of solubility problems and side reactions with the functional groups [158,159,160].
The Meldal group expected that a solid-phase approach would suffer less from these drawbacks, therefore, they undertook a study with Ser, Thr, and Tyr containing hexapeptides, hav-
3
Chemical Glycosylation Reactions
⊡ Scheme 25
fully carried out, partly by following known procedures [155,156]. Also an important Galβ
(1–4)GlcNAcβ(1–3)-L-Fuc moiety was prepared, which is part of O-linked chains of human
clotting factor IX [157].
More demanding is direct glycosylation of peptides, which was so far not very successful
because of solubility problems and side reactions with the functional groups [158,159,160].
The Meldal group expected that a solid-phase approach would suffer less from these drawbacks, therefore, they undertook a study with Ser, Thr, and Tyr containing hexapeptides, hav-