improved (P <0.05) the hatching efficiency, the strains 8', S33, S7, S1, S'10 and S'2 decreases the
load of gram-negative bacteria such that: Pseudomonas aeruginosa was removed by S33 and 8 ',
Enterobacter cloacae by S1, S'10 and S'2, Pasteurella pneumotropica by S7, S33 and 8' and on
the other hand the development of certain flora beneficial for Artemia, such as: the proliferation
of Stenotrophomonas maltophilia in the presence of the strain S2, 8 'and 3. The results of the
integration of the three probiotic strains to the two microalgae, revealed that the size of nauplius
fed by the chlorella inoculated with strain 8 'improved the two parameters TL and TF and the
size of nauplii fed by Spirulina inoculated by strain 8' and S33 improved the TF parameter. The
survival rate study was limited because commercial individuals with a short life span were used.
We find that the strains tested found in vitro appear to have probiotic effects in vivo in the
culture of Artemia. These beneficial effects must be studied in greater depth in order to elucidate
their mode of action.
Key words: Probiotic, Artemia, Enterococcus durans, Enterococcus faecuim and Psychrobacter
cibarius..
‫اﻟﻤﻠﺨﺺ‬
:
‫ﻓﻲ‬ ‫اﻟﺤﻲ‬ ‫اﻟﺘﻄﺒﯿﻖ‬ ‫إﻟﻰ‬ ‫اﻟﺤﺎﻟﻲ‬ ‫اﻟﻌﻤﻞ‬ ‫ﯾﮭﺪف‬
‫اﻷ‬ ‫ﺗﺮﺑﯿﺔ‬
‫رﺗﯿﻤﯿﺎ‬
‫ﻣﻦ‬
12
‫ذات‬ ‫ﺳﻼﻟﺔ‬
‫إ‬
‫ﺣﺘﻤﺎل‬
‫ﻓﺼﯿﻠﺔ‬ ‫إﻟﻰ‬ ‫ﺗﻨﺘﻤﻲ‬ ‫ﺑﺮوﺑﯿﻮﺗﯿﻚ‬
Enterococcus durans
‫و‬
Enterococcus faecium
‫و‬
cibarius
Psychrobacter
‫اﻟﻔﻘﺲ‬ ‫ﻛﻔﺎءة‬ ‫ﺗﺤﺴﯿﻦ‬ ‫أﺟﻞ‬ ‫ﻣﻦ‬
‫اﻟﻤﯿﻜﺮوﺑﯿﻮﻟﻮﺟﯿﺔ‬ ‫واﻟﺠﻮدة‬
‫ﺗﺮﺑﯿﺔ‬ ‫ﻟﻤﯿﺎه‬
‫اﻟﺤﯿﺎة‬ ‫ﻗﯿﺪ‬ ‫ﻋﻠﻰ‬ ‫واﻟﺒﻘﺎء‬ ‫اﻟﻨﻤﻮ‬ ‫ﻣﻌﺪل‬ ‫وﺗﺤﺴﯿﻦ‬ ‫ﻧﻮﺑﻠﯿﻮس‬
‫ﻟﻼ‬
‫ﺗﻢ‬ ‫اﻟﺘﻲ‬ ‫اﻟﻨﺘﺎﺋﺞ‬ ‫أظﮭﺮت‬ .‫رﺗﯿﻤﯿﺎ‬
‫ﻋﻠﯿﮭ‬ ‫اﻟﺤﺼﻮل‬
' ‫اﻟﺴﻼﻟﺘﯿﻦ‬ ‫أن‬ ‫ﺎ‬
8
‫و‬
S'2
) ‫ﻣﻠﺤﻮظ‬ ‫ﺑﺸﻜﻞ‬ ‫ﺗﺤﺴﻨﺖ‬ ‫ﻗﺪ‬
P
>
0.05
(
‫ﻣﻦ‬
' ‫اﻟﺴﻼﻻت‬ ، ‫اﻟﻔﻘﺲ‬ ‫ﻛﻔﺎءة‬
8
‫و‬
S33
‫و‬
S7
‫و‬
S1
‫و‬
S'10
‫و‬
S'2
‫إزاﻟﺔ‬ ‫ﺗﻤﺖ‬ :‫ﻣﺜﻞ‬ ‫اﻟﺒﻜﺘﯿﺮﯾﺎ‬ ‫ﺣﻤﻞ‬ ‫ﻣﻦ‬ ‫ﯾﻘﻠﻞ‬
Pseudomonas aeruginosa
‫ﺑﻮاﺳﻄﺔ‬
S33
‫و‬
'
8
،
Enterobacter cloacae
‫ﺑﻮاﺳﻄﺔ‬
S1
‫و‬
S'10
‫و‬
S'2
‫و‬
Pasteurella pneumotropica
‫ﺑﻮاﺳﻄﺔ‬
S7
‫و‬
S33
' ‫و‬
8
‫وﻣﻦ‬
‫ﺑﻌﺾ‬ ‫ﺗﻄﻮر‬ ‫أﺧﺮى‬ ‫ﻧﺎﺣﯿﺔ‬
‫اﻟﺒﻜﺘﯿﺮي‬
‫اﻟﻤﻔﯿﺪة‬
‫ﻟ‬
‫ﺳﻼﻟﺔ‬ ‫وﺟﻮد‬ ‫ﻓﻲ‬ ‫اﻟﻀﯿﻘﺔ‬ ‫اﻟﻤﯿﺘﻮﻓﯿﻠﯿﺎ‬ ‫اﻧﺘﺸﺎر‬ :‫ﻣﺜﻞ‬ ، ‫ﻸرﺗﯿﻤﯿﺎ‬
S2
' ،
8
‫و‬
3
‫ﻛﺸﻔﺖ‬ .
‫ﺣﺠﻢ‬ ‫أن‬ ‫اﻟﺪﻗﯿﻘﺔ‬ ‫اﻟﻄﺤﺎﻟﺐ‬ ‫إﻟﻰ‬ ‫ﺑﺮوﺑﯿﻮﺗﯿﻚ‬ ‫ﺳﻼﻻت‬ ‫ﺛﻼث‬ ‫ﺗﻜﺎﻣﻞ‬ ‫ﻧﺘﺎﺋﺞ‬
‫ﺑ‬ ‫اﻟﻤﻐﺬاة‬ ‫اﻻرﺗﯿﻤﯿﺎ‬
‫ﺎﻟﻜﻠﻮر‬
‫ﯾﻼ‬
‫ﺳﻼﻟﺔ‬ ‫ﻣﻊ‬ ‫اﻟﻤﻠﻘﺤﺔ‬
'
8
‫ﻛﻞ‬ ‫ﺗﺤﺴﯿﻦ‬
‫ﻣﻦ‬
‫إﻋﺪاد‬
‫ات‬
TL
‫و‬
TF
'،
8
‫و‬
S33
‫ﺳﺒﯿﺮوﻟﯿﻨﺎ‬ ‫ﻣﻊ‬ ‫ﺗﺴﺘﻜﻤﻞ‬ ‫ﺳﻼﻻت‬
‫ﻣﻌﻠﻤﺔ‬ ‫ﺗﺤﺴﻦ‬
TF
‫ﻓﻲ‬ ‫اﺧﺘﺒﺎرھﺎ‬ ‫ﺗﻢ‬ ‫اﻟﺘﻲ‬ ‫اﻟﺴﻼﻻت‬ ‫أن‬ ‫ﻧﺠﺪ‬ .
‫أﺟﻞ‬ ‫ﻣﻦ‬ ‫أﻛﺒﺮ‬ ‫ﺑﺘﻌﻤﻖ‬ ‫اﻟﻤﻔﯿﺪة‬ ‫اﻟﺘﺄﺛﯿﺮات‬ ‫ھﺬه‬ ‫دراﺳﺔ‬ ‫ﯾﺠﺐ‬ .‫ارﺗﯿﻤﯿﺎ‬ ‫ﺛﻘﺎﻓﺔ‬ ‫ﻓﻲ‬ ‫اﻟﺤﻲ‬ ‫اﻟﺠﺴﻢ‬ ‫ﻓﻲ‬ ‫ﺑﺮوﺑﯿﻮﺗﯿﻚ‬ ‫آﺛﺎر‬ ‫ﻟﮭﺎ‬ ‫أﻧﮭﺎ‬ ‫ﯾﺒﺪو‬ ‫اﻟﻤﺨﺘﺒﺮ‬
.‫ﻋﻤﻠﮭﺎ‬ ‫طﺮﯾﻘﺔ‬ ‫ﺗﻮﺿﯿﺢ‬
‫اﻟ‬
‫اﻟﻤﻔﺘﺎﺣﯿﺔ‬ ‫ﻜﻠﻤﺎت‬
:
،‫اﻷرﺗﯿﻤﯿﺎ‬ ، ‫اﻟﺒﺮوﺑﯿﻮﺗﯿﻚ‬
Enterococcus durans
،
Enterococcus faecium
‫و‬
cibarius
Psychrobacter
.