contaminated seawater and sediment is the prerequisite
for future bioremediation applications to the Algerian
coasts. The collection of Algerian hydrocarbon biodegraders obtained in this study could be exploited for
biotechnological applications also integrated into newly
designed, ready to use, biodegradable scaffold-bacteria
systems (Scaffaro et al. 2017).
Conflict of Interest
No conflict of interest declared.
References
Al-Awadhi, H., Dashti, N., Kansour, M., Sorkhoh, N. and
Radwan, S. (2012) Hydrocarbon-utilizing bacteria
associated with biofouling materials from offshore waters
of the Arabian Gulf. Int Biodeterior Biodegradation 69, 10–
16.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman,
D.J. (1990) Basic local alignment search tool. J Mol Biol
215, 403–410.
Atlas, R.M. (1981) Microbial degradation of petroleum
hydrocarbons: an environmental perspective. Microbiol Rev
45, 180.
Banat, I.M., Franzetti, A., Gandolfi, I., Bestetti, G., Martinotti,
M.G., Fracchia, L., Smyth, T.J. and Marchant, R. (2010)
Microbial biosurfactants production, applications and
future potential. Appl Microbial Biotechnol 87, 427–444.
Batista, S.B., Mounteer, A.H., Amorim, F.R. and Totola, M.R.
(2006) Isolation and characterization of biosurfactant/
bioemulsifier-producing bacteria from petroleum
contaminated sites. Bioresour Technol 97, 868–875.
Benmecheta, A. and Belkhir, L. (2016) Oil pollution in the
waters of Algeria. The Handbook of Environmental
Chemistry ed. Barcelo, D. and Kostianoy, A.G. pp. 1–16.
Berlin, Heidelberg: Springer.
Bodour, A.A. and Miller-Maier, R.M. (1998) Application of a
modified drop-collapse technique for surfactant
quantitation and screening of biosurfactant-producing
microorganisms. J Microbiol Methods 32, 273–280.
Cappello, S. and Yakimov, M.M. (2010) Alcanivorax. In
Handbook of Hydrocarbon and Lipid Microbiology ed.
Timmis, K.T. pp. 1737–1748. Berlin, Heidelberg: Springer.
Cappello, S., Caruso, G., Zampino, D., Monticelli, L.S.,
Maimone, G., Denaro, R., Tripodo, B., Troussellier, M.
et al. (2007) Microbial community dynamics during assays
of harbour oil spill bioremediation: a microscale
simulation study. J Appl Microbiol 102, 184–194.
Cappello, S., Volta, A., Santisi, S., Morici, C., Mancini, G.,
Quatrini, P., Genovese, M., Yakimov, M.M. et al. (2016)
Oil-degrading bacteria from a membrane bioreactor (BFMBR) system for treatment of saline oily waste:
Isolation, identification and characterization of the
biotechnological potential. Int Biodeterior Biodegradation
110, 235–244.
Catania, V., Santisi, S., Signa, G., Vizzini, S., Mazzola, A.,
Cappello, S., Yakimov, M.M. and Quatrini, P. (2015)
Intrinsic bioremediation potential of a chronically polluted
marine coastal area. Marine Poll Bull 99, 138–149.
Catania, V., Sara, G., Settanni, L. and Quatrini, P. (2016)
Bacterial communities in sediment of a Mediterranean
marine protected area. Can J Microbiol 63, 303–311.
Chaillan, F., Le Fl eche, A., Bury, E., Phantavong, Y.H., Grimont,
P., Saliot, A. and Oudot, J. (2004) Identification and
biodegradation potential of tropical aerobic hydrocarbondegrading microorganisms. Res Microbiol 155, 587–595.
Chandra, S., Sharma, R., Singh, K. and Sharma, A. (2013)
Application of bioremediation technology in the
environment contaminated with petroleum hydrocarbon.
Ann Microbiol 63, 417–431.
Chen, C., Liu, Q., Liu, C. and Yu, J. (2017) Effect of different
enrichment strategies on microbial community structure
in petroleum-contaminated marine sediment in Dalian,
China. Marine Poll Bull 117, 274–282.
China, B., Pirson, V. and Mainil, J. (1996) Typing of bovine
attaching and effacing Escherichia coli by multiplex in vitro
amplification of virulence-associated genes. Appl Environ
Microbiol 62, 3462–3465.
Daffonchio, D., Ferrer, M., Mapelli, F., Cherif, A., Lafraya, A.,
Malkawi, H.I., Yakimov, M.M., Abdel-Fattah, Y.R. et al.
(2013) Bioremediation of Southern Mediterranean oil
polluted sites comes of age. N Biotechnol 30, 743–748.
Das, N. and Chandran, P. (2011) Microbial degradation of
petroleum hydrocarbon contaminants: an overview.
Biotechnol Res Int 2011, 941810.
De Pasquale, C., Palazzolo, E., Piccolo, L.L. and Quatrini, P.
(2012) Degradation of long-chain n-alkanes in soil
microcosms by two actinobacteria. J Environ Sci Health A
Tox Hazard Subst Environ Eng 47, 374–381.
Dyksterhouse, S.E., Gray, J.P., Herwig, R.P., Lara, J.C. and
Staley, J.T. (1995) Cycloclasticus pugetii gen. nov., sp. nov.,
an aromatic hydrocarbon-degrading bacterium from
marine sediments. Int J Syst Evol Microbiol 45, 116–123.
EPA (1996) Method 3510c: Separatory Funnel Liquid-Liquid
Extraction Part of Test Methods for Evaluation Solid Waste,
Physical/Chemical Methods. Washington, DC: United
States Environmental Protection Agency.
Fathepure, B.Z. (2014) Recent studies in microbial degradation
of petroleum hydrocarbons in hypersaline environments.
Front Microbiol 5, 173.
Gao, X., Gao, W., Cui, Z., Han, B., Yang, P., Sun, C. and
Zheng, L. (2015) Biodiversity and degradation potential
of oil-degrading bacteria isolated from deep-sea
sediments of South Mid-Atlantic Ridge. Marine Poll Bull
97, 373–380.
Gauthier, M.J., Lafay, B., Christen, R., Fernandez, L.,
Acquaviva, M., Bonin, P. and Bertrand, J.C. (1992)
Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a
Journal of Applied Microbiology 126, 780--795 © 2018 The Society for Applied Microbiology
793
N. Djahnit et al.
The use of oil-degrading bacteria as an alternative for environmental remediation (bioremediation)
for future bioremediation applications to the Algerian
coasts. The collection of Algerian hydrocarbon biodegraders obtained in this study could be exploited for
biotechnological applications also integrated into newly
designed, ready to use, biodegradable scaffold-bacteria
systems (Scaffaro et al. 2017).
Conflict of Interest
No conflict of interest declared.
References
Al-Awadhi, H., Dashti, N., Kansour, M., Sorkhoh, N. and
Radwan, S. (2012) Hydrocarbon-utilizing bacteria
associated with biofouling materials from offshore waters
of the Arabian Gulf. Int Biodeterior Biodegradation 69, 10–
16.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman,
D.J. (1990) Basic local alignment search tool. J Mol Biol
215, 403–410.
Atlas, R.M. (1981) Microbial degradation of petroleum
hydrocarbons: an environmental perspective. Microbiol Rev
45, 180.
Banat, I.M., Franzetti, A., Gandolfi, I., Bestetti, G., Martinotti,
M.G., Fracchia, L., Smyth, T.J. and Marchant, R. (2010)
Microbial biosurfactants production, applications and
future potential. Appl Microbial Biotechnol 87, 427–444.
Batista, S.B., Mounteer, A.H., Amorim, F.R. and Totola, M.R.
(2006) Isolation and characterization of biosurfactant/
bioemulsifier-producing bacteria from petroleum
contaminated sites. Bioresour Technol 97, 868–875.
Benmecheta, A. and Belkhir, L. (2016) Oil pollution in the
waters of Algeria. The Handbook of Environmental
Chemistry ed. Barcelo, D. and Kostianoy, A.G. pp. 1–16.
Berlin, Heidelberg: Springer.
Bodour, A.A. and Miller-Maier, R.M. (1998) Application of a
modified drop-collapse technique for surfactant
quantitation and screening of biosurfactant-producing
microorganisms. J Microbiol Methods 32, 273–280.
Cappello, S. and Yakimov, M.M. (2010) Alcanivorax. In
Handbook of Hydrocarbon and Lipid Microbiology ed.
Timmis, K.T. pp. 1737–1748. Berlin, Heidelberg: Springer.
Cappello, S., Caruso, G., Zampino, D., Monticelli, L.S.,
Maimone, G., Denaro, R., Tripodo, B., Troussellier, M.
et al. (2007) Microbial community dynamics during assays
of harbour oil spill bioremediation: a microscale
simulation study. J Appl Microbiol 102, 184–194.
Cappello, S., Volta, A., Santisi, S., Morici, C., Mancini, G.,
Quatrini, P., Genovese, M., Yakimov, M.M. et al. (2016)
Oil-degrading bacteria from a membrane bioreactor (BFMBR) system for treatment of saline oily waste:
Isolation, identification and characterization of the
biotechnological potential. Int Biodeterior Biodegradation
110, 235–244.
Catania, V., Santisi, S., Signa, G., Vizzini, S., Mazzola, A.,
Cappello, S., Yakimov, M.M. and Quatrini, P. (2015)
Intrinsic bioremediation potential of a chronically polluted
marine coastal area. Marine Poll Bull 99, 138–149.
Catania, V., Sara, G., Settanni, L. and Quatrini, P. (2016)
Bacterial communities in sediment of a Mediterranean
marine protected area. Can J Microbiol 63, 303–311.
Chaillan, F., Le Fl eche, A., Bury, E., Phantavong, Y.H., Grimont,
P., Saliot, A. and Oudot, J. (2004) Identification and
biodegradation potential of tropical aerobic hydrocarbondegrading microorganisms. Res Microbiol 155, 587–595.
Chandra, S., Sharma, R., Singh, K. and Sharma, A. (2013)
Application of bioremediation technology in the
environment contaminated with petroleum hydrocarbon.
Ann Microbiol 63, 417–431.
Chen, C., Liu, Q., Liu, C. and Yu, J. (2017) Effect of different
enrichment strategies on microbial community structure
in petroleum-contaminated marine sediment in Dalian,
China. Marine Poll Bull 117, 274–282.
China, B., Pirson, V. and Mainil, J. (1996) Typing of bovine
attaching and effacing Escherichia coli by multiplex in vitro
amplification of virulence-associated genes. Appl Environ
Microbiol 62, 3462–3465.
Daffonchio, D., Ferrer, M., Mapelli, F., Cherif, A., Lafraya, A.,
Malkawi, H.I., Yakimov, M.M., Abdel-Fattah, Y.R. et al.
(2013) Bioremediation of Southern Mediterranean oil
polluted sites comes of age. N Biotechnol 30, 743–748.
Das, N. and Chandran, P. (2011) Microbial degradation of
petroleum hydrocarbon contaminants: an overview.
Biotechnol Res Int 2011, 941810.
De Pasquale, C., Palazzolo, E., Piccolo, L.L. and Quatrini, P.
(2012) Degradation of long-chain n-alkanes in soil
microcosms by two actinobacteria. J Environ Sci Health A
Tox Hazard Subst Environ Eng 47, 374–381.
Dyksterhouse, S.E., Gray, J.P., Herwig, R.P., Lara, J.C. and
Staley, J.T. (1995) Cycloclasticus pugetii gen. nov., sp. nov.,
an aromatic hydrocarbon-degrading bacterium from
marine sediments. Int J Syst Evol Microbiol 45, 116–123.
EPA (1996) Method 3510c: Separatory Funnel Liquid-Liquid
Extraction Part of Test Methods for Evaluation Solid Waste,
Physical/Chemical Methods. Washington, DC: United
States Environmental Protection Agency.
Fathepure, B.Z. (2014) Recent studies in microbial degradation
of petroleum hydrocarbons in hypersaline environments.
Front Microbiol 5, 173.
Gao, X., Gao, W., Cui, Z., Han, B., Yang, P., Sun, C. and
Zheng, L. (2015) Biodiversity and degradation potential
of oil-degrading bacteria isolated from deep-sea
sediments of South Mid-Atlantic Ridge. Marine Poll Bull
97, 373–380.
Gauthier, M.J., Lafay, B., Christen, R., Fernandez, L.,
Acquaviva, M., Bonin, P. and Bertrand, J.C. (1992)
Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a
Journal of Applied Microbiology 126, 780--795 © 2018 The Society for Applied Microbiology
793
N. Djahnit et al.
The use of oil-degrading bacteria as an alternative for environmental remediation (bioremediation)