TABLE 1
Experimental Design for Evaluation of PAAP*
Residence
Time
Days
Concentration,
%of
Sample
Samples·
5
Culture
Medium^
-
Lake
#1
Lake
#2
Lake
#3
Lake
#4
10
15
Batch
6
100
Enriched
Lake Water
5
100
Enriched
Lake Water
100
Enriched
Lake Water
100
Enriched
Lake Water
10
50
100
Enriched
Lake Water
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
5 reps./Exp.
* Experiment will be repeated a minimum of 4 times.
^Chemostat tests will be conducted at least 3 of the 4 residence times.
■^Lake water samples will be selected such that various stages of eutrophication will be covered.
4
Culture medium will be tested at concentrations of 10, 50 and 100% in both batch and chemostat.
5
10% PAAP medium nutrients in lake water.
6
Read 5 replicates per experiment.
T.E. Maloney, W.E. Miller and N.L. Blind