120
C. Wallis and J.L. Melnick
The present adsorbents consist of fibreglass and cellulose acetate filters 1 μ in porosity,
10 inches high, and woven to a thickness of 3/4 inch around a 1 inch effluent core. The
flow rates through these filters are 300 to 400 gallons per hour. In comparison with the
293 mm Millipore membrane previously used for concentrating virus (Wallis and Melnick,
1967), which has a surface of 486 cm
2 , the 10 inch textile adsorbent has a surface of
3250 cm
2 .
Ancillary equipment. The portable virus concentrator is mounted on a two-wheel
magnesium cart with a base of plywood 3/4 inch thick. The filter holders and related
equipment are shown in the figures and described below in detail.
The quick disconnects (QD) which allow the connection of two pieces of equipment
with a tubing (jumper) were purchased from the Snap Tite Corp., Union City, Penn. The
tubing with a male QD inserts instantly into the female QD attached to the equipment
and a 300 psi seal is obtained. Also, flow meters and additional textile filters can easily be
added to the system by QDs.
Check valves, which allow the passage of fluids only in one direction, and metering
valves which monitor the injection of salts and dyes into the running water, were
obtained from the Whitey Corp. (Thomas A. Read and Co., Houston, Texas). Multiport
valves which direct fluids in either of two directions were obtained from the Cabot Corp.,
Louisville, Ky.
The deep-well pump (Teel, model #3P513, obtained from the Dayton Electrical
Manufacturing Co., Chicago, Illinois) is capable of driving water at a rate of 300 to 600
GPH.
The gasoline driven electric generator is a Honda model # E 1500 which provides 1.5
kw (AC) for running the pump, the magnetic stirrers, and the other electrical equipment.
The 5-gallon pressure vessel was obtained from the Millipore Corporation, Bedford,
Mass.
The portable virus concentrator. Figure 1 is a top view of the equipment.
The numbered components indicate the continuity of flow. It is used as follows:
The generator is started, and the pump is removed from the bottom of the cart (Fig.
3). A 3/4 inch QD tubing is inserted into the pump and connected to the main inlet (1)*.
The electrical cord from the pump is plugged into the generator, and water is driven
through the system at a flow rate of 300 GPH.
When relatively clean water is under test, i.e. tap water, clean streams, etc. (as opposed
to sewage, murky water, etc.), filters in series of 5, 1 and 1 μ porosities are used, followed
by the virus adsorbent which also has a porosity of 1 μ. When sewage or murky water is
used, a pre-clarifying filter with a 100 μ porosity is added. This filter is contained in a
holder with a male QD on the outlet and a female QD on the inlet side, and inserted in
front of the 5 μ filter.
Prior to use, 5 gallons of test water is passed through the 5 and 1 μ polyester filters to
remove detergents left over from the manufacturing process. This water is discarded
through the effluent and not via the virus concentrators. Test water enters the system at
the inlet (1) and passes through the multiport valve. This directs the water through the
unit or when redirected will pass the water via a jumper to the main outlet (7). (This
redirection of water is used when equipment downstream requires repair or replacement.)
The water now passes the first clarifier(2) which is a 5 μ polyester or orlon filter which
only removes parti'culate matter, and does not affect the virus content of the water. The
*Number in parentjhesis refers to Figure 1.
C. Wallis and J.L. Melnick
The present adsorbents consist of fibreglass and cellulose acetate filters 1 μ in porosity,
10 inches high, and woven to a thickness of 3/4 inch around a 1 inch effluent core. The
flow rates through these filters are 300 to 400 gallons per hour. In comparison with the
293 mm Millipore membrane previously used for concentrating virus (Wallis and Melnick,
1967), which has a surface of 486 cm
2 , the 10 inch textile adsorbent has a surface of
3250 cm
2 .
Ancillary equipment. The portable virus concentrator is mounted on a two-wheel
magnesium cart with a base of plywood 3/4 inch thick. The filter holders and related
equipment are shown in the figures and described below in detail.
The quick disconnects (QD) which allow the connection of two pieces of equipment
with a tubing (jumper) were purchased from the Snap Tite Corp., Union City, Penn. The
tubing with a male QD inserts instantly into the female QD attached to the equipment
and a 300 psi seal is obtained. Also, flow meters and additional textile filters can easily be
added to the system by QDs.
Check valves, which allow the passage of fluids only in one direction, and metering
valves which monitor the injection of salts and dyes into the running water, were
obtained from the Whitey Corp. (Thomas A. Read and Co., Houston, Texas). Multiport
valves which direct fluids in either of two directions were obtained from the Cabot Corp.,
Louisville, Ky.
The deep-well pump (Teel, model #3P513, obtained from the Dayton Electrical
Manufacturing Co., Chicago, Illinois) is capable of driving water at a rate of 300 to 600
GPH.
The gasoline driven electric generator is a Honda model # E 1500 which provides 1.5
kw (AC) for running the pump, the magnetic stirrers, and the other electrical equipment.
The 5-gallon pressure vessel was obtained from the Millipore Corporation, Bedford,
Mass.
The portable virus concentrator. Figure 1 is a top view of the equipment.
The numbered components indicate the continuity of flow. It is used as follows:
The generator is started, and the pump is removed from the bottom of the cart (Fig.
3). A 3/4 inch QD tubing is inserted into the pump and connected to the main inlet (1)*.
The electrical cord from the pump is plugged into the generator, and water is driven
through the system at a flow rate of 300 GPH.
When relatively clean water is under test, i.e. tap water, clean streams, etc. (as opposed
to sewage, murky water, etc.), filters in series of 5, 1 and 1 μ porosities are used, followed
by the virus adsorbent which also has a porosity of 1 μ. When sewage or murky water is
used, a pre-clarifying filter with a 100 μ porosity is added. This filter is contained in a
holder with a male QD on the outlet and a female QD on the inlet side, and inserted in
front of the 5 μ filter.
Prior to use, 5 gallons of test water is passed through the 5 and 1 μ polyester filters to
remove detergents left over from the manufacturing process. This water is discarded
through the effluent and not via the virus concentrators. Test water enters the system at
the inlet (1) and passes through the multiport valve. This directs the water through the
unit or when redirected will pass the water via a jumper to the main outlet (7). (This
redirection of water is used when equipment downstream requires repair or replacement.)
The water now passes the first clarifier(2) which is a 5 μ polyester or orlon filter which
only removes parti'culate matter, and does not affect the virus content of the water. The
*Number in parentjhesis refers to Figure 1.