R. Billard e‘t J. Marcel, Ed. Aquaculture of Cyprinids, INRA, Paris, 1986.
PesËer sammaraes
DAILY CHANGES
IN THE GONADOTROPIN LEVELS AND RESPONSE OF CARP OOCYTES TO
PITUITARY HOMOGENATE.
K. BIENIÀRZ, w. POPEK, B. BRETON and P. EPLER, Dcpa&—
fmcn£ 05
cmd FÂÀhQ/LÏ.QA, Academy 05 AgMcuüu/æc, Ambmwwc @,
30—749
POLAND.
A total of
3 series of experiments were performed. ln series one 110 mature
carp females were used.
Blood was
sampled from the caudal blood vessels every
2 h
(different fish) or every 4h(the same fishes). Maturational gonadotr0pin
(mGtH) was radioimmunologically determined. ln series two, daily variations
in carp oocyte sensitivity to carp pituitary homogenate (Cph) were investi—
gated in vitro. Oocytes were taken from 12 females every4 h during 24 h, and
incubated in the medium with or without Cph. In addition, oocytes, taken from
another 4 females
in the morning and the evening, were placed in the medium
without Cph. Cph was added to the medium every 4 h during 24 h. After 24 h
incubation the percentage of oocytes after germinal vesicle breakdown was
counted.
ln series
three,
12 female
carp were injected with Cph in the mor—
ning and another 12 in the evening. The percentage of ovulated females was
established.
It was
found that mGtH and oocyte sensitivity to cph in vitro
and in vivo in mature female carp changed significantly over 24 h.
JOINT ACTION OF CARP PITUITARY HOMOGENATE AND HUMAN CHORIONIC GONADOTROPIN
(HCG) IN CARP OOCYTE MATURATION AND OVULATION. IN VITRO AND IN VIVO STUDY.
P. EPLER, M. SOKOLOWSKÀ, w. POPEK, and K. BIENIARZ, Depaäïmenä 06 IchÉhy0bÀ0—
Æogy and FÂ/3hQJÏÀQÀ, Academy 05 AgMcuüuc,
6, 30—149 Kaakow—
Myd£n£hi, POLAND.
In vitro study showed that HCG at a dose of IOOIU/ml did not cause the
percentage of mature oocytes (follicles) to increase compared to the control
incubations, but HCG together with carp pituitary homogenate (Cph), at ratios
of 6/4,5/5 and 4/6 for doses of 60, 50, 40 lU/ml of HCG and 40, 50, 60 ug/ml
Cph gave a higher mean percentage of mature oocytes than Cph alone at a dose
of
100 ug/ml. In vivo experiments were carried out on 200 adult female carp.
The use of HCG and Cph at ratios of 6/4, 5/5 and 4/6 and a dose Of 10 times
higher than in the in vitro experiment, in two injections, did not increase
the percentage of the mature oocytes or the number of ovulated females in
comparison to the groups injected with the same dose of cph alone. Class1cal
hypophysation induced ovulation in the majority of injected females (60—802).
The results indicate the ineffectiveness of HCG used with cph in order to
decrease the amount of Cph in the artificial spawning of carp.
CARP SPERM, A NEW MODEL FOR STUDYING SPERM MOTILITY REGUDATION.
M.P. COSSON,
J.L. GATTI, R. CHRISTEN, and R. BILLARD. CNRS, UA 577; V4££€6Æa”ChQ'ÔUË“MQË,
06230 FRANCE.
Cypriuid fish spermatozoa are immotile in the semen. Moti1ity is induced upon
SP8Wning in fresh water but ceases rapidly probably due to the swe111ug
of
the sperm under low external osmotic pressure (O.P). Our analys1s Of the
aCtivation of motility shows that in carp sperm the O.P. plays a centrallîole.
The sperm remain quiescent When the O.P. is above
200 mOsm and become
fu
y
aCtivated when it is less than 150 mOsm : ident1cal results are obtained when
PesËer sammaraes
DAILY CHANGES
IN THE GONADOTROPIN LEVELS AND RESPONSE OF CARP OOCYTES TO
PITUITARY HOMOGENATE.
K. BIENIÀRZ, w. POPEK, B. BRETON and P. EPLER, Dcpa&—
fmcn£ 05
cmd FÂÀhQ/LÏ.QA, Academy 05 AgMcuüu/æc, Ambmwwc @,
30—749
POLAND.
A total of
3 series of experiments were performed. ln series one 110 mature
carp females were used.
Blood was
sampled from the caudal blood vessels every
2 h
(different fish) or every 4h(the same fishes). Maturational gonadotr0pin
(mGtH) was radioimmunologically determined. ln series two, daily variations
in carp oocyte sensitivity to carp pituitary homogenate (Cph) were investi—
gated in vitro. Oocytes were taken from 12 females every4 h during 24 h, and
incubated in the medium with or without Cph. In addition, oocytes, taken from
another 4 females
in the morning and the evening, were placed in the medium
without Cph. Cph was added to the medium every 4 h during 24 h. After 24 h
incubation the percentage of oocytes after germinal vesicle breakdown was
counted.
ln series
three,
12 female
carp were injected with Cph in the mor—
ning and another 12 in the evening. The percentage of ovulated females was
established.
It was
found that mGtH and oocyte sensitivity to cph in vitro
and in vivo in mature female carp changed significantly over 24 h.
JOINT ACTION OF CARP PITUITARY HOMOGENATE AND HUMAN CHORIONIC GONADOTROPIN
(HCG) IN CARP OOCYTE MATURATION AND OVULATION. IN VITRO AND IN VIVO STUDY.
P. EPLER, M. SOKOLOWSKÀ, w. POPEK, and K. BIENIARZ, Depaäïmenä 06 IchÉhy0bÀ0—
Æogy and FÂ/3hQJÏÀQÀ, Academy 05 AgMcuüuc,
6, 30—149 Kaakow—
Myd£n£hi, POLAND.
In vitro study showed that HCG at a dose of IOOIU/ml did not cause the
percentage of mature oocytes (follicles) to increase compared to the control
incubations, but HCG together with carp pituitary homogenate (Cph), at ratios
of 6/4,5/5 and 4/6 for doses of 60, 50, 40 lU/ml of HCG and 40, 50, 60 ug/ml
Cph gave a higher mean percentage of mature oocytes than Cph alone at a dose
of
100 ug/ml. In vivo experiments were carried out on 200 adult female carp.
The use of HCG and Cph at ratios of 6/4, 5/5 and 4/6 and a dose Of 10 times
higher than in the in vitro experiment, in two injections, did not increase
the percentage of the mature oocytes or the number of ovulated females in
comparison to the groups injected with the same dose of cph alone. Class1cal
hypophysation induced ovulation in the majority of injected females (60—802).
The results indicate the ineffectiveness of HCG used with cph in order to
decrease the amount of Cph in the artificial spawning of carp.
CARP SPERM, A NEW MODEL FOR STUDYING SPERM MOTILITY REGUDATION.
M.P. COSSON,
J.L. GATTI, R. CHRISTEN, and R. BILLARD. CNRS, UA 577; V4££€6Æa”ChQ'ÔUË“MQË,
06230 FRANCE.
Cypriuid fish spermatozoa are immotile in the semen. Moti1ity is induced upon
SP8Wning in fresh water but ceases rapidly probably due to the swe111ug
of
the sperm under low external osmotic pressure (O.P). Our analys1s Of the
aCtivation of motility shows that in carp sperm the O.P. plays a centrallîole.
The sperm remain quiescent When the O.P. is above
200 mOsm and become
fu
y
aCtivated when it is less than 150 mOsm : ident1cal results are obtained when