—
v.
ARTIFICIAL INSEMINATION
…
,
.
…_In spite
of
the
vàst practical experience accumulated so far somepama—
metersof the actual technique of artificial insemination are not
such are
and pH, of the diluent— and the optimal sperm
A diluent based on
the mineral
composition of the seminal fluid is
since in
carp potassium is harmful to the eggs.
A
saline
solution
of NaCl-45
mM, KCl 5
Tris 20 mM,
to be;a_better diluent than water,.pr the
non—buffered» Woynarovichïs
allows a dilution rate of
(fig. 8). Preliminary results show that the maximum is obtained by mixing
:
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Z:CI«
Fig, BChæges1n
fertilization
tion
in
and-sperm dilu+
.
tion" rates.
Right:
comparison of ‘woynar0vich's solution
water
‘ï‘-'Â=” Éf-…'.«…>ŒÊQ)‘@and Dil
inseminati°n
5
Sperm(1o—5çrÿ
onevolume
After
the
ë3h<>uld
&
few: minutes. and then the st1cky .egügs are degummed
( 133%
NaCl 44: g/l )—,…
,
As…;an
Lsmi£lkgâmay;
uîse
£MŒ‘.®l
that faS.tifkaneâS
an: be '
PFÊYBn”°3@àW£: mxmg theeggsîor 39 mmm freshmlk ‘dilutèd
'diluent or Water.’
“
::Mikôdi‘ñà and
(198,03)
Ipî‘QP°-$ed
the use of a diluent‘for artificial insemination composed of 3 g/l of NaClhdggthqthtd tgA
°fthebl°°hemlstW°f
auL» of:… thecortmal u…%lQ30tion and ,o;£
thehardemngproœsswwldhelp t®impr0vethis procedUre
‘
.
=
-
,
«mn; t…… f…l «= …
1
Should
mixççäwith
=
5*mM',
Tris 20 mM,
pH
1nwh1ch spermd11ut10nrate
In
these ‘cbn‘dition
around
is 25
than the value
reported
'