124
C. Weil et et
In
the non ovulated fish,
17œ 208 OH P levels were low varying between
undetectable value
and O,5ng/ml. In the partially ovulated fish, a low rise
in
17d 208 OH P was observed between 18 and 21 hours, followed by a decrease
by 24
hours
when
ovulation was monitored.
On the
other hand higher levels
were
monitored
in
ovulating
fish
but the highest value was 7ng/ml, value
much lower
than
that
one
indicated by LEVAVI—ZERMONSKY and YARON (summary
this volume).
These
authors
report a dramatic (111ng/ml) but transient in—
crease
followed
by
GV breakdownn, corroborating the idea that 17Π208 DH P
is
involved
in
oocyte maturation. Furthermore during spontaneous ovulation
transient increase
of different progestins (progesterone, 17œ OH P, 17m 208
OH P)
have been recently described in goldfish
(KAGAWA et al.
1983). Since
these
increases
are
transitory, higher values of 17Π208 DH P may have been
attained between the
sampling periods in our experiment.
Hormonal profiles and spermiation
In the
literature, no data are available on circulating GtH and steroid
levels
following
hypophysation.
In
a
work
conducted
in our
laboratory,
(figure 2)
plasma
GtH,
androgens'
and
11—ketotestosterone were monitored
every 3 hours for a 2H hour period following a single injection of partially
purified carp
gonadotropin
(100pg/kg).
Collectable milt volume as well as
spermatocrit were
measured
before
the
injection and 12 and 24 hours after
the injection.
ËIZ
‘5
80
°\o
':
8
1
E'
5
\
70
°°
a
4
se
";
‘
0
50
22
200
U
t
‘\
U
E [8
L" ‘\
ISO %
ë
!
‘*l
&
ÎJ4
:
Ë '0
"
80
E
S‘
@
î
{
I
E‘
;
\””I \—B 40 ;
€
2
€
?
3
6
9
12
IS
l8
2l
24
GÎH
.
hrs post ùüecon
_
Figure 2 : Ëormonal changes and collected milt volume after hypophysation.
X
î_s.e.
n
:
8
'
The treatment induced a large increase in GtH levels (6üng/ml) 3 and 6 hours
after the
injection.
The
levels
decreased gradually from 9 to 24 hours to
îâîîî
dbY‘
hours.
These circulating levels are much higher than
terone Îîîïîse fîïîlng
Spontaneous spawn1ng
(ANONYMOUS 1978).
11
ketotestos—
owed the same profile than GtH with an increase the first
C. Weil et et
In
the non ovulated fish,
17œ 208 OH P levels were low varying between
undetectable value
and O,5ng/ml. In the partially ovulated fish, a low rise
in
17d 208 OH P was observed between 18 and 21 hours, followed by a decrease
by 24
hours
when
ovulation was monitored.
On the
other hand higher levels
were
monitored
in
ovulating
fish
but the highest value was 7ng/ml, value
much lower
than
that
one
indicated by LEVAVI—ZERMONSKY and YARON (summary
this volume).
These
authors
report a dramatic (111ng/ml) but transient in—
crease
followed
by
GV breakdownn, corroborating the idea that 17Π208 DH P
is
involved
in
oocyte maturation. Furthermore during spontaneous ovulation
transient increase
of different progestins (progesterone, 17œ OH P, 17m 208
OH P)
have been recently described in goldfish
(KAGAWA et al.
1983). Since
these
increases
are
transitory, higher values of 17Π208 DH P may have been
attained between the
sampling periods in our experiment.
Hormonal profiles and spermiation
In the
literature, no data are available on circulating GtH and steroid
levels
following
hypophysation.
In
a
work
conducted
in our
laboratory,
(figure 2)
plasma
GtH,
androgens'
and
11—ketotestosterone were monitored
every 3 hours for a 2H hour period following a single injection of partially
purified carp
gonadotropin
(100pg/kg).
Collectable milt volume as well as
spermatocrit were
measured
before
the
injection and 12 and 24 hours after
the injection.
ËIZ
‘5
80
°\o
':
8
1
E'
5
\
70
°°
a
4
se
";
‘
0
50
22
200
U
t
‘\
U
E [8
L" ‘\
ISO %
ë
!
‘*l
&
ÎJ4
:
Ë '0
"
80
E
S‘
@
î
{
I
E‘
;
\””I \—B 40 ;
€
2
€
?
3
6
9
12
IS
l8
2l
24
GÎH
.
hrs post ùüecon
_
Figure 2 : Ëormonal changes and collected milt volume after hypophysation.
X
î_s.e.
n
:
8
'
The treatment induced a large increase in GtH levels (6üng/ml) 3 and 6 hours
after the
injection.
The
levels
decreased gradually from 9 to 24 hours to
îâîîî
dbY‘
hours.
These circulating levels are much higher than
terone Îîîïîse fîïîlng
Spontaneous spawn1ng
(ANONYMOUS 1978).
11
ketotestos—
owed the same profile than GtH with an increase the first