For the algal bodies cultured over one week, 47 wt% oil was obtained, with
neutral lipid content of 32.9%, which accounted for 80% of oil stores.
Fatty acid composition has been known to vary greatly with culturing conditions. In the case of DA0580, proportions of various fatty acids changed with
alterations in culturing conditions (such as temperature), with content of 0.8% C 16 –
C 26 fatty acid hydrocarbons and 0.3% squalene. The strain showed few of the
long-chain unsaturated fatty acids responsible for degradation during repurposing
for biodiesel fuel, and because palmitoleic methyl esters have a low melting point of
0.5 °C, solidification does not occur at low temperatures. DA0580 oils thus have
Fig. 9.12 Growth and oil accumulation in Navicula sp. JPCC DA 0580 (Nile red; white portions
are oil droplets)
Table 9.10 Fatty acid composition for oils extracted from Navicula sp. JPCC DA0580
Biodiesel fuel
suitable
EU norm under for 12%
Total
C 14: 0
C 16:
0
C 16:
1
C 18:
0
C 18:
1
C 18:
2
C 18:
3
C 20:
5
Navicula sp. 4JPCC
DA 0580
3.1
38.6 51.6 0.0 2.4 0.0 n.d. 4.3 100
Chlorella sp.
0.0
26
2.0 6.0 32
18
16
0.0 100
340
9 Marine Bioenergy Production