Table 6.2 Developing synthetic medium for major seaweeds (in 100 ml)
ASP 1
ASP 2 ASP 6
ASP 7 ASP 12 KDX
ASS 1
Sodium chloride (NaCl)
2.4 g
1.8 g
2.4 g
2.5 g 2.8 g
1.9 g
2.5 g
Sodium sulfate (Na 2 SO 4 )
–
–
–
–
–
0.32 g
–
Magnesium sulfate
(MgSO4 · 7H2O)
0.6 g
0.5 g
0.8 g
0.9 g 0.7 g
–
1.0 g
Magnesium chloride
(MgCl 2· 7H 2 O)
0.45 g –
–
–
0.4 g
0.87 g
–
Potassium chloride (KCl)
60 mg 60 mg 70 mg 70 mg 70 mg –
70 mg
Calcium chloride (CaCl 2 )
40 mg 10 mg 15 mg 30 mg 40 mg 50 mg 30 mg
Sodium nitrate (NaNO 3 )
10 mg 5 mg 30 mg 5 mg 10 mg 8.5 mg 10 mg
Ammonium sulfate
[(NH4) 2 SO 4 )]
–
–
–
–
–
0.66 mg –
Dipotassium phosphate
(K 2 HPO 4 )
2 mg
0.5 mg –
–
–
–
–
Tripotassium phosphate
(K 3 PO 4 )
–
–
–
–
1 mg
–
–
Monosodium phosphate
(Na 2 HPO4 · H2O)
–
–
–
–
–
0.7 g
–
Sodium silicate
(Na 2 SiO 3· 9H 2 O)
2.5 mg 15 mg 7 mg
7 mg 15 mg –
–
Sodium carbonate
(NaCO 3· H 2 O)
–
3 mg –
–
–
–
–
Sodium bicarbonate
(NaHCO 3 )
–
–
–
–
–
8.8 mg 10 mg
Sodium glycerophosphate
–
–
10 mg 2 mg 1 mg
0.14 mg 2 mg
Iron [Fe (as Cl
−
)]
–
50 µg –
–
–
–
–
Metal mixture P II
a
1 ml
3 ml
–
3 ml
1 ml
–
–
Metal mixture S II
b
–
–
–
–
1 ml
–
–
Metal mixture P8
c
–
–
1 ml
–
–
–
–
Metal mixture (ASS)
d
–
–
–
–
–
–
1 ml
Vitamin B 12
0.02 µg 0.2 µg 0.05 µg 0.1 µg 0.02 µg –
–
Biotin
–
–
–
–
0.1 µg –
–
Thiamine hydrochloride
–
–
–
–
10 µg –
–
Mixed vitamin solution S3
e
–
1 ml
–
1 ml
–
–
–
Mixed vitamin solution 8A
f
0.05 ml –
0.1 ml –
–
–
–
Mixed vitamin solution
(ASS)
g
–
–
–
–
–
–
0.1 ml
KDS solution
h
–
–
–
–
–
1 ml
–
KDTM solution
i
–
–
–
–
–
1 ml
–
Triaminomethane
0.1 g
0.1 g
0.1 g
0.1 g 0.1 g
–
–
(continued)
6.3 Tissue Culture
151