3 Surface-enhanced Raman Scattering ...
43
Another example of successful application of SERRS for detecting the presence
and measuring activity of the enzyme is the work of Ruan et al. [125], in which
SERRS spectrum of a blue indigo dye produced by oxidation and dimerization of
5-bromo-4-chloro-3-indolyl phosphate was utilized for monitoring activity of alkaline phosphatase.
detection of dNA is one of the most important tasks in bioanalysis. to date,
numerous SERS measurements of dNA utilizing different SERS supports have
been reported. Experiments can be roughly divided into two groups. In the first
group, called label-free method, dNA is directly detected with the SERS nanoprobes. For example, halas group reported SERS spectra of dNA oligonucleotides
and found that they are selectively dominated by the vibrational modes of adenine
[126]. the authors also demonstrated the capability of SERS in investigating the
interaction of dNA with various molecules (in this case—cisplatin). While direct
SERS measurements for dNA are rather scarce, there are many reports on the indirect SERS experiments. this approach to dNA detection consists of monitoring
the SERS spectrum of reporter molecules (usually dyes) that are attached to the metallic nanoparticles. this kind of SERS experiments for dNA is more extensively
described in sect. 4.2.1.
Fig. 3.7 SERS enzyme immunoassay system (a) and enzyme catalyzed reaction (b). (Reproduced
from Ref. [123] with kind permission of the American Chemical Society)
43
Another example of successful application of SERRS for detecting the presence
and measuring activity of the enzyme is the work of Ruan et al. [125], in which
SERRS spectrum of a blue indigo dye produced by oxidation and dimerization of
5-bromo-4-chloro-3-indolyl phosphate was utilized for monitoring activity of alkaline phosphatase.
detection of dNA is one of the most important tasks in bioanalysis. to date,
numerous SERS measurements of dNA utilizing different SERS supports have
been reported. Experiments can be roughly divided into two groups. In the first
group, called label-free method, dNA is directly detected with the SERS nanoprobes. For example, halas group reported SERS spectra of dNA oligonucleotides
and found that they are selectively dominated by the vibrational modes of adenine
[126]. the authors also demonstrated the capability of SERS in investigating the
interaction of dNA with various molecules (in this case—cisplatin). While direct
SERS measurements for dNA are rather scarce, there are many reports on the indirect SERS experiments. this approach to dNA detection consists of monitoring
the SERS spectrum of reporter molecules (usually dyes) that are attached to the metallic nanoparticles. this kind of SERS experiments for dNA is more extensively
described in sect. 4.2.1.
Fig. 3.7 SERS enzyme immunoassay system (a) and enzyme catalyzed reaction (b). (Reproduced
from Ref. [123] with kind permission of the American Chemical Society)