505
Chapter 17
Vibrational Microspectroscopy for Analysis
of Atherosclerotic Arteries
T.P. Wróbel, A. Fedorowicz, Ł. Mateuszuk, E. Maślak, A. Jasztal,
S. Chlopicki and K.M. Marzec
K.m. marzec () T.P. Wróbel · A. Fedorowicz · Ł. Mateuszuk · E. Maślak · A. Jasztal ·
S. Chlopicki
Jagiellonian Centre for Experimental therapeutics (JCEt),
Jagiellonian university, Bobrzynskiego 14,
30-348, Krakow, Poland
e-mail: marzec@chemia.uj.edu.pl
t.P. Wróbel
Faculty of Chemistry, Jagiellonian university, Ingardena 3,
30-060, Krakow, Poland
S. Chlopicki
department of Experimental Pharmacology, Chair of Pharmacology, Jagiellonian university,
medical College, grzegorzecka 16,
31-531, Krakow, Poland
Abstract this Chapter describes the fundamental principles of atherosclerotic
plaque formation along with features detectable by histology. the main focus is
on reviewing the use of Raman and IR spectroscopy in the study of atherosclerotic
arteries. the analysis of the experimental results is possible only with the application of mathematical and statistical tools of analysis, i.e. chemometrics, which are
also described. this chapter proves that chemometrics allows us to create a link
between IR and Raman spectroscopy and their application in chemistry.
Keywords Infrared spectroscopy • Raman spectroscopy • Chemical imaging •
Chemometrics • Atherosclerosis • Atherosclerotic plaque • Cardiovascular disease
17.1 Introduction
the desire of scientists to have an insight into the biochemical composition of a
structure of tissues without the need for labelling, has initiated a number of studies utilizing the application of various spectroscopic techniques [1]. Raman and
IR spectroscopies were found to be among the most successful for studying the
differences in the relative concentrations of specific groups of compounds. they
have been used successfully in many studies where the goal was to differentiate
between normal and pathological tissue [2]. the use of Fourier transform Infrared Spectroscopy (Ft-IR) in biomedical sciences has increased significantly in
m. Baranska (ed.), Optical Spectroscopy and Computational Methods in Biology and
Medicine, Challenges and Advances in Computational Chemistry and Physics 14,
doI 10.1007/978-94-007-7832-0_17, © Springer Science+Business media dordrecht 2014
Chapter 17
Vibrational Microspectroscopy for Analysis
of Atherosclerotic Arteries
T.P. Wróbel, A. Fedorowicz, Ł. Mateuszuk, E. Maślak, A. Jasztal,
S. Chlopicki and K.M. Marzec
K.m. marzec () T.P. Wróbel · A. Fedorowicz · Ł. Mateuszuk · E. Maślak · A. Jasztal ·
S. Chlopicki
Jagiellonian Centre for Experimental therapeutics (JCEt),
Jagiellonian university, Bobrzynskiego 14,
30-348, Krakow, Poland
e-mail: marzec@chemia.uj.edu.pl
t.P. Wróbel
Faculty of Chemistry, Jagiellonian university, Ingardena 3,
30-060, Krakow, Poland
S. Chlopicki
department of Experimental Pharmacology, Chair of Pharmacology, Jagiellonian university,
medical College, grzegorzecka 16,
31-531, Krakow, Poland
Abstract this Chapter describes the fundamental principles of atherosclerotic
plaque formation along with features detectable by histology. the main focus is
on reviewing the use of Raman and IR spectroscopy in the study of atherosclerotic
arteries. the analysis of the experimental results is possible only with the application of mathematical and statistical tools of analysis, i.e. chemometrics, which are
also described. this chapter proves that chemometrics allows us to create a link
between IR and Raman spectroscopy and their application in chemistry.
Keywords Infrared spectroscopy • Raman spectroscopy • Chemical imaging •
Chemometrics • Atherosclerosis • Atherosclerotic plaque • Cardiovascular disease
17.1 Introduction
the desire of scientists to have an insight into the biochemical composition of a
structure of tissues without the need for labelling, has initiated a number of studies utilizing the application of various spectroscopic techniques [1]. Raman and
IR spectroscopies were found to be among the most successful for studying the
differences in the relative concentrations of specific groups of compounds. they
have been used successfully in many studies where the goal was to differentiate
between normal and pathological tissue [2]. the use of Fourier transform Infrared Spectroscopy (Ft-IR) in biomedical sciences has increased significantly in
m. Baranska (ed.), Optical Spectroscopy and Computational Methods in Biology and
Medicine, Challenges and Advances in Computational Chemistry and Physics 14,
doI 10.1007/978-94-007-7832-0_17, © Springer Science+Business media dordrecht 2014