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carbohydrates moieties, lipid and protein content, cholesterol, collagen, phosphodiester-containing macromolecules and others [115–125]. the changes are associated
with the type of the tumour and its origin. It should be noted that various multivariate approaches have been employed for sensitive and selective classification of the
grade and origin of brain tumours, i.e. uhCA [120, 122], K-means [118, 121, 123],
SImCA (Soft Independent modeling of Class Analogy) [119, 123], PCA [123, 125],
LdA [116–118, 124] and their combinations.
Fig. 15.8 a Spectra from different cell layers prior to Rmie-EmSC. b After Rmie-EmSC. c h&E
stained section of the normal cervical epithelium. d map generated by integrating the area beneath
the amide I mode. e uhCA performed on data before Rmie-EmSC. f uhCA performed on data
after Rmie-EmSC
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