213
Chapter 8
Two-Dimensional Mid-Infrared Correlation
Spectroscopy in Protein Research
Bogusława Czarnik-Matusewicz and Young Mee Jung
B. Czarnik-matusewicz ()
Faculty of Chemistry, University of Wrocław, F. Joliot-Curie 14,
50–383, Wrocław, Poland
e-mail: boguslawa.czarnik@gmail.com
Y. m. Jung
department of Chemistry, Kangwon National university,
Chuncheon, 200–701 Korea
Abstract Representative results showing the position and strength of two-dimensional (2d) correlation spectroscopy in protein research are surveyed in this
article. Special emphasis is placed on infrared spectroscopy. different types of
external perturbations that are particularly useful for exploring properties of proteins are discussed. most promising developments in 2d correlation spectroscopy are
demonstrated through results obtained for protein systems. the aim of this article
has been to present 2d correlation spectroscopy as a simple method that significantly improves information about protein structure gained from infrared spectra.
Keywords 2D correlation spectroscopy • Infrared spectroscopy • Protein structure •
Amide I • Heterospectral correlation
8.1 Introduction
Combined with “protein research,” the following three phrases could confuse
a reader who is unfamiliar with the subjects and the terminology used: “two-dimensional infrared (2d IR) spectroscopy”, “Correlation-2d IR spectroscopy” and
“two-dimensional correlation spectroscopy (2dCoS)”. Additionally, the apparent
similarities in the graphical presentations of the results from each of these spectroscopies could be very misleading. generally, all of the phrases relate to methods that
have been inspired by the two-dimensional techniques first developed in the field
of nuclear magnetic resonance. however, 2d IR spectroscopies and 2d correlation spectroscopies are fundamentally different because they are based on nonlinear
and linear interactions of electromagnetic waves with matter, respectively, as was
underlined by Noda and ozaki [1]. According to [2], the method of 2d IR spectroscopy concerns a nonlinear interaction of light and matter in which measurements
m. Baranska (ed.), Optical Spectroscopy and Computational Methods in Biology and
Medicine, Challenges and Advances in Computational Chemistry and Physics 14,
doI 10.1007/978-94-007-7832-0_8, © Springer Science+Business media dordrecht 2014
Chapter 8
Two-Dimensional Mid-Infrared Correlation
Spectroscopy in Protein Research
Bogusława Czarnik-Matusewicz and Young Mee Jung
B. Czarnik-matusewicz ()
Faculty of Chemistry, University of Wrocław, F. Joliot-Curie 14,
50–383, Wrocław, Poland
e-mail: boguslawa.czarnik@gmail.com
Y. m. Jung
department of Chemistry, Kangwon National university,
Chuncheon, 200–701 Korea
Abstract Representative results showing the position and strength of two-dimensional (2d) correlation spectroscopy in protein research are surveyed in this
article. Special emphasis is placed on infrared spectroscopy. different types of
external perturbations that are particularly useful for exploring properties of proteins are discussed. most promising developments in 2d correlation spectroscopy are
demonstrated through results obtained for protein systems. the aim of this article
has been to present 2d correlation spectroscopy as a simple method that significantly improves information about protein structure gained from infrared spectra.
Keywords 2D correlation spectroscopy • Infrared spectroscopy • Protein structure •
Amide I • Heterospectral correlation
8.1 Introduction
Combined with “protein research,” the following three phrases could confuse
a reader who is unfamiliar with the subjects and the terminology used: “two-dimensional infrared (2d IR) spectroscopy”, “Correlation-2d IR spectroscopy” and
“two-dimensional correlation spectroscopy (2dCoS)”. Additionally, the apparent
similarities in the graphical presentations of the results from each of these spectroscopies could be very misleading. generally, all of the phrases relate to methods that
have been inspired by the two-dimensional techniques first developed in the field
of nuclear magnetic resonance. however, 2d IR spectroscopies and 2d correlation spectroscopies are fundamentally different because they are based on nonlinear
and linear interactions of electromagnetic waves with matter, respectively, as was
underlined by Noda and ozaki [1]. According to [2], the method of 2d IR spectroscopy concerns a nonlinear interaction of light and matter in which measurements
m. Baranska (ed.), Optical Spectroscopy and Computational Methods in Biology and
Medicine, Challenges and Advances in Computational Chemistry and Physics 14,
doI 10.1007/978-94-007-7832-0_8, © Springer Science+Business media dordrecht 2014