175
these are two very important aspects of ECd as a non-degrading method of fast
conformational analysis of proteins and nucleic acids. What is more, a short time of
a Cd measurement makes it a suitable tool for time-dependent studies. Electronic
Cd and CPL are particularly valuable when the structure of a biomolecule changes
in time because of external stimulus (change of solvent, temperature, or complex
formation). this makes Cd an important tool in drug discovery (when the stimulus is a pharmacologically active molecule), and investigations of such structural
changes as protein denaturation, amyloid formation, or dNA melting.
References
1. Biot JB (1817) mem Acad Roy Sci Inst France 2:41
2. djerassi C (1960) optical Rotatory dispersion. (mcgraw-hill, New York,)
3. Snatzke g (1968) Angew Chem Internat Edit 7:14
4. haidinger W (1847) Poggendorfs Annalen 70, 531
5. Cotton Am (1896) Ann Chim Physique 8:347
6. Laur P (2012) Comprehensive chiroptical spectroscopy: volume 1—instrumentation, methodologies, and theoretical simulations. Wiley, hoboken. (Chap. the first decades after the
discovery of Cd and oRd by Aime Cotton in 1895)
7. Sreerama N, Woody RW (2000) Circular dichroism: Principles and applications. Wiley, New
York, pp 601–620 (Chap. Circular dichroism of Peptides and Proteins)
8. Cantor C, Schimmel P (1980) Biophysical Chemistry, Part 2: techniques for the Study of
Biological Structure and Function. W.h. Freeman and Company, New York
9. Rodger A, Norden B (1997) Circular dichroism and Linear dichroism. oxford university
Press, oxford. (oxford Chemistry masters)
10. Woody RW (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in
stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley,
hoboken. (Chap. Electronic Circular dichroism of Proteins)
11. toniolo C, Formaggio F, Woody RW (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stereochemical analysis of synthetic compounds, natural products, and
biomolecules. Wiley, hoboken. (Chap. Electronic Circular dichroism of Peptides)
12. Kypr J, Kejnovska I, Bednarova K, vorlickova m (2012) "Comprehensive chiroptical spectroscopy: volume 1—applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley, hoboken. (Chap. Electronic Circular dichroism
Spectroscopy of Nucleic Acids)
13. gray dm (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stere- ochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley,
hoboken. (Chap. Circular dichroism of Protein-Nucleic Acid Interactions)
14. Ellestad gA (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley,
hoboken. (Chap. drug and Natural Product Binding to Nucleic Acids Analyzed by Electronic Circular dichroism)
15. Bertucci C, Pistolozzi m (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley, hoboken. (Chap. Electronic Circular dichroism in drug discovery)
16. Kelly S, Price N (1997) BBA-Protein Struct m 1338:161
17. Bulheller B, Rodger A, hirst J (2007) Phys Chem Chem Phys 9:2020
18. Kelly S, Jess t, Price N (2005) BBA-Proteins Proteom 1751:119
19. miles A, Wallace B (2006) Chem Soc Rev 35:39
6 Electronic Circular dichroism Spectroscopy in Structural Analysis …
these are two very important aspects of ECd as a non-degrading method of fast
conformational analysis of proteins and nucleic acids. What is more, a short time of
a Cd measurement makes it a suitable tool for time-dependent studies. Electronic
Cd and CPL are particularly valuable when the structure of a biomolecule changes
in time because of external stimulus (change of solvent, temperature, or complex
formation). this makes Cd an important tool in drug discovery (when the stimulus is a pharmacologically active molecule), and investigations of such structural
changes as protein denaturation, amyloid formation, or dNA melting.
References
1. Biot JB (1817) mem Acad Roy Sci Inst France 2:41
2. djerassi C (1960) optical Rotatory dispersion. (mcgraw-hill, New York,)
3. Snatzke g (1968) Angew Chem Internat Edit 7:14
4. haidinger W (1847) Poggendorfs Annalen 70, 531
5. Cotton Am (1896) Ann Chim Physique 8:347
6. Laur P (2012) Comprehensive chiroptical spectroscopy: volume 1—instrumentation, methodologies, and theoretical simulations. Wiley, hoboken. (Chap. the first decades after the
discovery of Cd and oRd by Aime Cotton in 1895)
7. Sreerama N, Woody RW (2000) Circular dichroism: Principles and applications. Wiley, New
York, pp 601–620 (Chap. Circular dichroism of Peptides and Proteins)
8. Cantor C, Schimmel P (1980) Biophysical Chemistry, Part 2: techniques for the Study of
Biological Structure and Function. W.h. Freeman and Company, New York
9. Rodger A, Norden B (1997) Circular dichroism and Linear dichroism. oxford university
Press, oxford. (oxford Chemistry masters)
10. Woody RW (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in
stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley,
hoboken. (Chap. Electronic Circular dichroism of Proteins)
11. toniolo C, Formaggio F, Woody RW (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stereochemical analysis of synthetic compounds, natural products, and
biomolecules. Wiley, hoboken. (Chap. Electronic Circular dichroism of Peptides)
12. Kypr J, Kejnovska I, Bednarova K, vorlickova m (2012) "Comprehensive chiroptical spectroscopy: volume 1—applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley, hoboken. (Chap. Electronic Circular dichroism
Spectroscopy of Nucleic Acids)
13. gray dm (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stere- ochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley,
hoboken. (Chap. Circular dichroism of Protein-Nucleic Acid Interactions)
14. Ellestad gA (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley,
hoboken. (Chap. drug and Natural Product Binding to Nucleic Acids Analyzed by Electronic Circular dichroism)
15. Bertucci C, Pistolozzi m (2012) Comprehensive chiroptical spectroscopy: volume 2—applications in stereochemical analysis of synthetic compounds, natural products, and biomolecules. Wiley, hoboken. (Chap. Electronic Circular dichroism in drug discovery)
16. Kelly S, Price N (1997) BBA-Protein Struct m 1338:161
17. Bulheller B, Rodger A, hirst J (2007) Phys Chem Chem Phys 9:2020
18. Kelly S, Jess t, Price N (2005) BBA-Proteins Proteom 1751:119
19. miles A, Wallace B (2006) Chem Soc Rev 35:39
6 Electronic Circular dichroism Spectroscopy in Structural Analysis …