58
2 Holographic Microscopy of Phase and Diffuse Objects …
Since 1978 in the Institute of Neurology, Neurosurgery and Physiotherapy of the
Ministry of Health of the BSSR, the employees of the team “Coherent-optical studies
of medico-biological systems” under supervision and on the initiative of the author L.
V. Tanin along with the employees of the first neurologic and physiotherapy departments under the supervision of Professor I. P. Antonov and Professor G. K. Nedzved
carried out laser therapy by low-intensity laser radiation during the treatment of
patients with peripheral nervous system diseases including intravascular laser blood
irradiation (ILBI) [4, 6, 11, 12, 16, 18, 21, 26–28, 30– 40, 75, 77].
At the same time, the necessity appeared of more detailed study of structural
and functional characteristics of neuromuscular tissue under the influence of laser
radiation, magnetic field, increased pressure in connection with its medicinal use as
the mean providing the formation of stable adaptive process at different organization
levels [15, 17, 77].
Thus, the development and integration of the method of holographic interference
microscopy with electrophysiological control is of utmost interest. It is used for the
studies of the structure and function changes in isolated excited specimen of nerve
and muscular tissue being in the conditions of increased pressure. When solving this
problem, we based on the following conditions: noninvasivity, high measurement
sensitivity, dynamic studies and the possibility of simultaneous electrophysiological
control. There are some peculiarities when using coherent-optical methods in the
studies of nerve and muscular tissues. These peculiarities are connected with the
multiple light scattering in the object, its microstructure changes, the use of increasing
optics under the considerable thickness of optical windows of hyperbaric cells, the
deformation of cells useful capacity under pressure application. It generates the need
for the developing additional experimental processes that allow using holographic
methods in the present conditions [15, 17, 70, 71, 72]. These studies are covered in
detail in Sect. 2.3.
Primarily, as a technical object, a semiconductor crystal of laser diode was chosen.
It was chosen for the purpose of developing the method of holographic microscopy
with regard to the studies of diffusely reflective medico-biological microobjects. This
object was used to train the design features of the holographic interference microscope in reflected light, and the holographic method was adapted to the interference
studies of diffusive objects. As a result, the integrated study of thermal characteristics of semiconductor crystal faces of laser diode was carried out. The studies of
the deformation of laser diodes, which worked in continuous and pulsed regimes
and which were carried out along with the employees of the Laboratory of Semiconductor Optics of the Institute of Physics of the NAS of Belarus using the method of
holographic interference microscopy, are also of independent scientific and practical
interest [55–69].
2 Holographic Microscopy of Phase and Diffuse Objects …
Since 1978 in the Institute of Neurology, Neurosurgery and Physiotherapy of the
Ministry of Health of the BSSR, the employees of the team “Coherent-optical studies
of medico-biological systems” under supervision and on the initiative of the author L.
V. Tanin along with the employees of the first neurologic and physiotherapy departments under the supervision of Professor I. P. Antonov and Professor G. K. Nedzved
carried out laser therapy by low-intensity laser radiation during the treatment of
patients with peripheral nervous system diseases including intravascular laser blood
irradiation (ILBI) [4, 6, 11, 12, 16, 18, 21, 26–28, 30– 40, 75, 77].
At the same time, the necessity appeared of more detailed study of structural
and functional characteristics of neuromuscular tissue under the influence of laser
radiation, magnetic field, increased pressure in connection with its medicinal use as
the mean providing the formation of stable adaptive process at different organization
levels [15, 17, 77].
Thus, the development and integration of the method of holographic interference
microscopy with electrophysiological control is of utmost interest. It is used for the
studies of the structure and function changes in isolated excited specimen of nerve
and muscular tissue being in the conditions of increased pressure. When solving this
problem, we based on the following conditions: noninvasivity, high measurement
sensitivity, dynamic studies and the possibility of simultaneous electrophysiological
control. There are some peculiarities when using coherent-optical methods in the
studies of nerve and muscular tissues. These peculiarities are connected with the
multiple light scattering in the object, its microstructure changes, the use of increasing
optics under the considerable thickness of optical windows of hyperbaric cells, the
deformation of cells useful capacity under pressure application. It generates the need
for the developing additional experimental processes that allow using holographic
methods in the present conditions [15, 17, 70, 71, 72]. These studies are covered in
detail in Sect. 2.3.
Primarily, as a technical object, a semiconductor crystal of laser diode was chosen.
It was chosen for the purpose of developing the method of holographic microscopy
with regard to the studies of diffusely reflective medico-biological microobjects. This
object was used to train the design features of the holographic interference microscope in reflected light, and the holographic method was adapted to the interference
studies of diffusive objects. As a result, the integrated study of thermal characteristics of semiconductor crystal faces of laser diode was carried out. The studies of
the deformation of laser diodes, which worked in continuous and pulsed regimes
and which were carried out along with the employees of the Laboratory of Semiconductor Optics of the Institute of Physics of the NAS of Belarus using the method of
holographic interference microscopy, are also of independent scientific and practical
interest [55–69].