5.15 Studies of Spectral Properties of Radiation Scattered by Blood …
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of ILBI (10 sessions within 10 days). Radiation was conducted with the help of the
device for ILBI (semiconductor laser with λ = 850 nm). Power of radiation amounted
to 2 mW.
Along with these experiments based on the given technique, absorption spectra
were registered twice and concentration of protein was defined in blood preparation
in seven experimental animals, which constituted control group.
For the quantitative estimation of the concentration of oxyhemoglobin in blood
preparation of experimental animals before and after the conduction of ILBI and
normalization of the results of measurements, there were used the calculated ratios
of amplitudes of characteristic local extremums of the registered spectral curves of
absorption to the concentration of protein in the blood probes under study, and also
coefficients Q 1 and Q 2 , corresponding to:
Q 1 = Ka λ 1 − Ka λ 2 ; Q 2 = Ka λ 3 − Ka λ 2 ,
where Ka λ 1 = A 542 /B; Ka λ 2 = A 560 /B; and Ka λ 3 = A 577 /B are the ratios of amplitudes of local extremums of absorption spectra at the wavelengths of 542 and 560
and 577 nm to the protein concentration in the blood probe under study, respectively.
The results of the conducted measurements and calculations of the coefficients
Q 1 and Q 2 , suggested for the estimation of the degree of oxygenation of blood
preparation, are presented in Tables 5.10, 5.11 and 5.12.
With the help of three-wavelength spectrophotometric method, it was found out
that the conduction of ILBI therapy by the radiation of semiconductor laser at partial
ischemia of sciatic nerve in experimental animals leads to 55% change of the calculated coefficient Q 1 that corresponds to the difference between the ratios of amplitudes of local extremums of absorption spectra of blood preparation at wavelengths
of 542 and 560 nm to the concentration in the probes under study, and to the 57%
change of coefficient Q 2 , corresponding to the difference between ratios of amplitudes of local extremums of spectral curves at wavelengths of 542 and 577 nm to the
concentration of protein in the probes under study, in comparison with the analogous
coefficients in animals of control group.
Thereby, as a result of conducted studies it was found out that the size of spectral
intensity changes considerably as a result of distinctive reflective features of skin of
different people. However, the relative value of local extremums for the wavelengths
λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577 nm remains practically stable for all the
respondents.
The conducted studies of spectral characteristics of human blood preparation
in vivo for the range of 200–700 nm at different levels of oxygenation showed that
there are the same local extremums λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577
nm at spectral curves of blood preparation, relative values of which considerably
change during the change of the level of oxygenation. The curve of dependence
between oxygenation level of human blood preparation and relative values of local
extremums at corresponding spectral curves was made, by which it is possible to
determine the blood preparation oxygenation level.
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of ILBI (10 sessions within 10 days). Radiation was conducted with the help of the
device for ILBI (semiconductor laser with λ = 850 nm). Power of radiation amounted
to 2 mW.
Along with these experiments based on the given technique, absorption spectra
were registered twice and concentration of protein was defined in blood preparation
in seven experimental animals, which constituted control group.
For the quantitative estimation of the concentration of oxyhemoglobin in blood
preparation of experimental animals before and after the conduction of ILBI and
normalization of the results of measurements, there were used the calculated ratios
of amplitudes of characteristic local extremums of the registered spectral curves of
absorption to the concentration of protein in the blood probes under study, and also
coefficients Q 1 and Q 2 , corresponding to:
Q 1 = Ka λ 1 − Ka λ 2 ; Q 2 = Ka λ 3 − Ka λ 2 ,
where Ka λ 1 = A 542 /B; Ka λ 2 = A 560 /B; and Ka λ 3 = A 577 /B are the ratios of amplitudes of local extremums of absorption spectra at the wavelengths of 542 and 560
and 577 nm to the protein concentration in the blood probe under study, respectively.
The results of the conducted measurements and calculations of the coefficients
Q 1 and Q 2 , suggested for the estimation of the degree of oxygenation of blood
preparation, are presented in Tables 5.10, 5.11 and 5.12.
With the help of three-wavelength spectrophotometric method, it was found out
that the conduction of ILBI therapy by the radiation of semiconductor laser at partial
ischemia of sciatic nerve in experimental animals leads to 55% change of the calculated coefficient Q 1 that corresponds to the difference between the ratios of amplitudes of local extremums of absorption spectra of blood preparation at wavelengths
of 542 and 560 nm to the concentration in the probes under study, and to the 57%
change of coefficient Q 2 , corresponding to the difference between ratios of amplitudes of local extremums of spectral curves at wavelengths of 542 and 577 nm to the
concentration of protein in the probes under study, in comparison with the analogous
coefficients in animals of control group.
Thereby, as a result of conducted studies it was found out that the size of spectral
intensity changes considerably as a result of distinctive reflective features of skin of
different people. However, the relative value of local extremums for the wavelengths
λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577 nm remains practically stable for all the
respondents.
The conducted studies of spectral characteristics of human blood preparation
in vivo for the range of 200–700 nm at different levels of oxygenation showed that
there are the same local extremums λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577
nm at spectral curves of blood preparation, relative values of which considerably
change during the change of the level of oxygenation. The curve of dependence
between oxygenation level of human blood preparation and relative values of local
extremums at corresponding spectral curves was made, by which it is possible to
determine the blood preparation oxygenation level.