596
J. Jaisawal et al.
6 Various Applications of AFM-FS and AFM-SMFS:
AFM-FS has been used for various applications, as shown in Fig. 4. It has
been utilized to investigate the protein folding/unfolding and polynucleotides with
repeated units, as well as the intermolecular interactions or molecule–materials interfaces (MI) [25–27]. It can also be utilized to study the cellular mechanics besides
imaging different types of cells and biomolecules [28, 29]. Besides this, AFM-SMFS
can also be used for both quantitative analysis and detection. The advancements in the
field of AFM-FS pursued by some well-established research groups were published in
previous literature [30–36]. For example, the progress in AFM-SMFS have led to the
application of this technique on polyproteins using force extension and force-clamp
modes. These two main operation modes were explained very nicely by Dougan
group [30]. The fundamentals and implementations of AFM-SMFS for evaluating
the molecular elasticity and localization of single polysaccharides and proteins by
Fig. 4 Schematic representation of various applications of AFM-FS and AFM-SMFS. Adapted
with permission from [37]. Copyright 2016 Royal Society of Chemistry
J. Jaisawal et al.
6 Various Applications of AFM-FS and AFM-SMFS:
AFM-FS has been used for various applications, as shown in Fig. 4. It has
been utilized to investigate the protein folding/unfolding and polynucleotides with
repeated units, as well as the intermolecular interactions or molecule–materials interfaces (MI) [25–27]. It can also be utilized to study the cellular mechanics besides
imaging different types of cells and biomolecules [28, 29]. Besides this, AFM-SMFS
can also be used for both quantitative analysis and detection. The advancements in the
field of AFM-FS pursued by some well-established research groups were published in
previous literature [30–36]. For example, the progress in AFM-SMFS have led to the
application of this technique on polyproteins using force extension and force-clamp
modes. These two main operation modes were explained very nicely by Dougan
group [30]. The fundamentals and implementations of AFM-SMFS for evaluating
the molecular elasticity and localization of single polysaccharides and proteins by
Fig. 4 Schematic representation of various applications of AFM-FS and AFM-SMFS. Adapted
with permission from [37]. Copyright 2016 Royal Society of Chemistry