Fluorescence Spectrometry
Dharmendra Pratap Singh, Sanjeev R. Inamdar, and Sandeep Kumar
Abstract Fluorescence spectrometry (FLS) is a fundamental toolbox that provides
in-depth information about the electronic transitions and interactions in a molecular system. With this technique, one can assay the state of a physical, chemical, or
biological system by studying their photophysical properties. The electronic transitions or interactions in a particular molecular system are monitored by analyzing the
changes in the fluorescent optical properties and their life-times. In this chapter, we
have included the fundamental principles of fluorescence spectrometry along with the
experimental and working aspects. The concepts of steady-state and time-resolved
fluorescence spectroscopy and the modern development in this regime have been discussed in detail. All the aspects of FLS for characterizing the soft-nano composites
have been comprised and explained. The future perspectives and modern applications of FLS in physical, chemical and medical sciences along with the bio-imaging
have been covered within the scope of this chapter.
Keywords Fluorescence spectrometry · Energy transfer mechanism ·
Fluorescence spectroscopy for mesogenic-nanocomposites · FRET ·
Time-resolved fluorescence
D. P. Singh (B)
Université du Líttoral Côte d’Opale, UR 4476, UDSMM, Unité de Dynamique et Structure des
Matériaux Moléculaires, 62228 Calais, France
Department of Industrial Engineering, EIL Côte d’Opale La Malassise, CS 50109, 62968
Longuenesse, France
e-mail: dharmendra.singh@univ-littoral.fr
S. R. Inamdar
Laser Spectroscopy Programme, Department of Physics, Karnatak University, Dharwad 580003,
India
e-mail: him_lax3@yahoo.com
S. Kumar
Soft Condensed Matter, Raman Research Institute, Sadashiva Nagar, Bengaluru 560080, India
e-mail: skumar@rri.res.in
Department of Chemistry, Nitte Meenakshi Institute of Technology (NMIT), Yelahanka,
Bengaluru 560064, India
© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
D. K. Singh et al. (eds.), Modern Techniques of Spectroscopy, Progress in Optical Science
and Photonics 13, https://doi.org/10.1007/978-981-33-6084-6_17
431
Dharmendra Pratap Singh, Sanjeev R. Inamdar, and Sandeep Kumar
Abstract Fluorescence spectrometry (FLS) is a fundamental toolbox that provides
in-depth information about the electronic transitions and interactions in a molecular system. With this technique, one can assay the state of a physical, chemical, or
biological system by studying their photophysical properties. The electronic transitions or interactions in a particular molecular system are monitored by analyzing the
changes in the fluorescent optical properties and their life-times. In this chapter, we
have included the fundamental principles of fluorescence spectrometry along with the
experimental and working aspects. The concepts of steady-state and time-resolved
fluorescence spectroscopy and the modern development in this regime have been discussed in detail. All the aspects of FLS for characterizing the soft-nano composites
have been comprised and explained. The future perspectives and modern applications of FLS in physical, chemical and medical sciences along with the bio-imaging
have been covered within the scope of this chapter.
Keywords Fluorescence spectrometry · Energy transfer mechanism ·
Fluorescence spectroscopy for mesogenic-nanocomposites · FRET ·
Time-resolved fluorescence
D. P. Singh (B)
Université du Líttoral Côte d’Opale, UR 4476, UDSMM, Unité de Dynamique et Structure des
Matériaux Moléculaires, 62228 Calais, France
Department of Industrial Engineering, EIL Côte d’Opale La Malassise, CS 50109, 62968
Longuenesse, France
e-mail: dharmendra.singh@univ-littoral.fr
S. R. Inamdar
Laser Spectroscopy Programme, Department of Physics, Karnatak University, Dharwad 580003,
India
e-mail: him_lax3@yahoo.com
S. Kumar
Soft Condensed Matter, Raman Research Institute, Sadashiva Nagar, Bengaluru 560080, India
e-mail: skumar@rri.res.in
Department of Chemistry, Nitte Meenakshi Institute of Technology (NMIT), Yelahanka,
Bengaluru 560064, India
© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
D. K. Singh et al. (eds.), Modern Techniques of Spectroscopy, Progress in Optical Science
and Photonics 13, https://doi.org/10.1007/978-981-33-6084-6_17
431