208
2
Alfred Wegener Institute, Helmholtz Centre for Polar
and Marine Research, Am Handelshafen 12, D-27570
Bremerhaven, Germany
*corresponding author: zurhelle@uni-bremen.de
Keywords: DOM, Metabolite profiling, Spring bloom
Helgoland
Remineralization of algae biomass by heterotrophic bacteria plays an important role in carbon cycling. Teeling and
co-workers observed the secondary bloom of planktonic bacteria succeeding a phytoplankton bloom in the German Bight
(Teeling et al. 2012, Science 336: 608–611). These bacterioplankta have specialized carbohydrate-active enzymes that
allow them to decompose organic matter released by microalgae (Teeling et al. 2012) resulting in recurring bacterioplankton succession patterns largely governed by
substrate-induced forcing (Teeling et al. 2016, eLife 5:
e11888). However, qualitative and quantitative shifts of
organic matter (i.e., bacterial substrate) during and after an
algal bloom are still poorly understood therefore limiting our
understanding of community succession and biodiversity
patterns. Here, I will present a study of how the dissolved
organic matter (DOM) composition changed over a phytoplankton bloom in spring 2016 at Helgoland in the German
Bight. Utilizing nuclear magnetic resonance spectroscopy
(NMR), my data shows clear shifts in DOM constituents as
well as quantitative bursts of DOM depended on abundance
shifts of microalgae. My findings are the first step to linking
the chemical specificity of phytoplankton-derived DOM
with the subsequent bloom of substrate-specialized bacteria.
4.3 Abstracts of Poster Presentations
4.3.1 Identification of Microalgal Cyclic Imines
and Their Potential Ecological Roles
Joyce Nieva
1*
, Boris Koch
1
, Jan Tebben
1
, Bernd Krock
1
,
Urban Tillman
1
, Ulf Bickmeyer
1
1
Alfred Wegener Institute, Am Handelshafen 12, 27570
Bremerhaven, Germany
*corresponding author: joyce.nieva@awi.de
Keywords: Cyclic imines, Chemical elucidation,
Ecological role, Dissolved organic matter
Cyclic imines constitute the growing group of toxins produced by dinoflagellates. To date, of the two (2) main groups
of cyclic imines, only 11 spirolides and 4 gymnodimines are
structurally elucidated. With a high number of cyclic imines
that are to be identified, this study aims to determine the
other congeners and elucidate its structure, function to the
organism and its ecological impact. Cyclic imines from
Alexandrium ostenfeldii strains isolated from the Netherlands,
Norway and Argentina will be chemically elucidated through
combined use of LC-MS/MS and 1D- and 2D NMR techniques. The potency and structure activity relationship of
cyclic imines on acetylcholine receptors (AChR) will be
determined through cell line assays. For its role as an allelophatic compound, trace metal binding studies and grazing
experiments will be conducted to determine the role of cyclic
imines as a ligand for micronutrient uptake and as a deterrent
compound, respectively. Further, this study will investigate
the presence of the cyclic imines in the dissolved organic
matter (DOM) by analysis of DOM samples from different
oceanographic expeditions: MSM65 (Arctic/Greenland), HE
492 (Svalbard) and HE 514 (British Channel, Celtic Sea,
North Sea). Expected results of this study include the determination of the novel cyclic imines, mechanism of action
and their possible ecological role.
5 Reading the Book of Life – Omics
as a Universal Tool Across Disciplines
Jan D. Brüwer
1,2
and Hagen Buck-Wiese
2
1
Red Sea Research Center, Division of Biological and
Environmental Science and Engineering (BESE), King
Abdullah University of Science and Technology (KAUST),
Thuwal 23955-6900, Saudi Arabia
2
Faculty of Biology and Chemistry, University of Bremen,
P.O. Box 330440, 28334 Bremen, Germany
5.1 Call for Abstracts
From the poles to the tropics and from shallow waters to the
deep sea: The marine environment is the greatest and most
diverse system on the planet. As diverse as the habitats are
the disciplines of marine sciences. Understanding the universal molecular languages, has revolutionized the information
available to almost all of them. These advances in omics generate ever more data, demanding data mining and allow specific research question. We invite researchers across
disciplines to submit their research to introduce cutting-edge
methodologies, reveal insights achievable thanks to omics,
and inspire approaches across the board.
5.2 Abstracts of Oral Presentations
5.2.1 PhyloFlash – Metagenomics, Microbial
Molecular Ecology and a New Age of Discovery
Harald Gruber-Vodicka
1*
, Elmar Pruesse
2
, and Brandon
K. B. Seah
1
1
Max Planck Institute for Marine Microbiology, Bremen,
Germany
2
University of Colorado Denver, Colorado, U.S.A
*invited speaker, corresponding author: hgruber@mpibremen.de
Appendices
2
Alfred Wegener Institute, Helmholtz Centre for Polar
and Marine Research, Am Handelshafen 12, D-27570
Bremerhaven, Germany
*corresponding author: zurhelle@uni-bremen.de
Keywords: DOM, Metabolite profiling, Spring bloom
Helgoland
Remineralization of algae biomass by heterotrophic bacteria plays an important role in carbon cycling. Teeling and
co-workers observed the secondary bloom of planktonic bacteria succeeding a phytoplankton bloom in the German Bight
(Teeling et al. 2012, Science 336: 608–611). These bacterioplankta have specialized carbohydrate-active enzymes that
allow them to decompose organic matter released by microalgae (Teeling et al. 2012) resulting in recurring bacterioplankton succession patterns largely governed by
substrate-induced forcing (Teeling et al. 2016, eLife 5:
e11888). However, qualitative and quantitative shifts of
organic matter (i.e., bacterial substrate) during and after an
algal bloom are still poorly understood therefore limiting our
understanding of community succession and biodiversity
patterns. Here, I will present a study of how the dissolved
organic matter (DOM) composition changed over a phytoplankton bloom in spring 2016 at Helgoland in the German
Bight. Utilizing nuclear magnetic resonance spectroscopy
(NMR), my data shows clear shifts in DOM constituents as
well as quantitative bursts of DOM depended on abundance
shifts of microalgae. My findings are the first step to linking
the chemical specificity of phytoplankton-derived DOM
with the subsequent bloom of substrate-specialized bacteria.
4.3 Abstracts of Poster Presentations
4.3.1 Identification of Microalgal Cyclic Imines
and Their Potential Ecological Roles
Joyce Nieva
1*
, Boris Koch
1
, Jan Tebben
1
, Bernd Krock
1
,
Urban Tillman
1
, Ulf Bickmeyer
1
1
Alfred Wegener Institute, Am Handelshafen 12, 27570
Bremerhaven, Germany
*corresponding author: joyce.nieva@awi.de
Keywords: Cyclic imines, Chemical elucidation,
Ecological role, Dissolved organic matter
Cyclic imines constitute the growing group of toxins produced by dinoflagellates. To date, of the two (2) main groups
of cyclic imines, only 11 spirolides and 4 gymnodimines are
structurally elucidated. With a high number of cyclic imines
that are to be identified, this study aims to determine the
other congeners and elucidate its structure, function to the
organism and its ecological impact. Cyclic imines from
Alexandrium ostenfeldii strains isolated from the Netherlands,
Norway and Argentina will be chemically elucidated through
combined use of LC-MS/MS and 1D- and 2D NMR techniques. The potency and structure activity relationship of
cyclic imines on acetylcholine receptors (AChR) will be
determined through cell line assays. For its role as an allelophatic compound, trace metal binding studies and grazing
experiments will be conducted to determine the role of cyclic
imines as a ligand for micronutrient uptake and as a deterrent
compound, respectively. Further, this study will investigate
the presence of the cyclic imines in the dissolved organic
matter (DOM) by analysis of DOM samples from different
oceanographic expeditions: MSM65 (Arctic/Greenland), HE
492 (Svalbard) and HE 514 (British Channel, Celtic Sea,
North Sea). Expected results of this study include the determination of the novel cyclic imines, mechanism of action
and their possible ecological role.
5 Reading the Book of Life – Omics
as a Universal Tool Across Disciplines
Jan D. Brüwer
1,2
and Hagen Buck-Wiese
2
1
Red Sea Research Center, Division of Biological and
Environmental Science and Engineering (BESE), King
Abdullah University of Science and Technology (KAUST),
Thuwal 23955-6900, Saudi Arabia
2
Faculty of Biology and Chemistry, University of Bremen,
P.O. Box 330440, 28334 Bremen, Germany
5.1 Call for Abstracts
From the poles to the tropics and from shallow waters to the
deep sea: The marine environment is the greatest and most
diverse system on the planet. As diverse as the habitats are
the disciplines of marine sciences. Understanding the universal molecular languages, has revolutionized the information
available to almost all of them. These advances in omics generate ever more data, demanding data mining and allow specific research question. We invite researchers across
disciplines to submit their research to introduce cutting-edge
methodologies, reveal insights achievable thanks to omics,
and inspire approaches across the board.
5.2 Abstracts of Oral Presentations
5.2.1 PhyloFlash – Metagenomics, Microbial
Molecular Ecology and a New Age of Discovery
Harald Gruber-Vodicka
1*
, Elmar Pruesse
2
, and Brandon
K. B. Seah
1
1
Max Planck Institute for Marine Microbiology, Bremen,
Germany
2
University of Colorado Denver, Colorado, U.S.A
*invited speaker, corresponding author: hgruber@mpibremen.de
Appendices