Analysis of Microbial Isolate
from Contaminated Street Food
with a Potential to Degrade Food Waste
S. Swathi Priya, C. Shanmuga Priya, and J. Maria Shyla
Abstract Contaminated food stuff is a potential source of bacteria with interest
encompassing mirid attributes prompting their exploitation in assorted applications including environment domain like degradation of waste and discarded food.
Street food has bacterial contamination like Salmonella sp., Staphylococcus aureus,
Escherichia coli, Bacillus sp., Mostly non-significance bacteria are present in the
street foods but still point to environmental contamination, in this work food samples
were collected and serially diluted, isolated confirmed using different media. The
isolation and identification process were done to know and characterise the bacteria
via 16 s rRNA gene sequence and the DNA these further elucidated via BLAST
search engine.
Keywords Bacteria · DNA sequence · Bacillus. sp · Micro-organisms · Street food
1 Introduction
Bacteria are ubiquitous in nature. Gram-positive bacteria that are encountered in food
samples; S. aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Listeria
monocytogenes, Bacillus etc. Gram-negative bacteria; Escherichia coli, Salmonella,
Shigella, and other Enterobacteriaceae, Pseudomonas etc. (Gdoura-Ben Amor et al.
2018) There are 30–40 million bacterial cells in a gram of food. Genus Bacillus
is of prominence especially when taken in consideration the significance for its
role in the biodegradation of fatty materials in food wastes, as it is armed with an
array of important producing capabilities like lipase- and biosurfactant-producing
species, also they are capable of creating endospores that empowers them resistant
to conflicting; extreme environmental situations, hence they have a special niche in
the biological handling of pollutants and xenobiotics. Bacillus spp. along with other
fat-degrading microorganisms like as Acinetobacter spp. and/or Pseudomonas spp.
have been isolated from an assortment of sources, including contaminated food stuffs
S. Swathi Priya · C. Shanmuga Priya · J. Maria Shyla (B)
Department of IT, PSGR Krishnammal College for Women, Coimbatore, TamilNadu, India
e-mail: mariatommy07@gmail.com
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2021
M. Prashanthi et al. (eds.), Bioremediation and Green Technologies, Environmental
Science and Engineering, https://doi.org/10.1007/978-3-030-64122-1_16
235
from Contaminated Street Food
with a Potential to Degrade Food Waste
S. Swathi Priya, C. Shanmuga Priya, and J. Maria Shyla
Abstract Contaminated food stuff is a potential source of bacteria with interest
encompassing mirid attributes prompting their exploitation in assorted applications including environment domain like degradation of waste and discarded food.
Street food has bacterial contamination like Salmonella sp., Staphylococcus aureus,
Escherichia coli, Bacillus sp., Mostly non-significance bacteria are present in the
street foods but still point to environmental contamination, in this work food samples
were collected and serially diluted, isolated confirmed using different media. The
isolation and identification process were done to know and characterise the bacteria
via 16 s rRNA gene sequence and the DNA these further elucidated via BLAST
search engine.
Keywords Bacteria · DNA sequence · Bacillus. sp · Micro-organisms · Street food
1 Introduction
Bacteria are ubiquitous in nature. Gram-positive bacteria that are encountered in food
samples; S. aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Listeria
monocytogenes, Bacillus etc. Gram-negative bacteria; Escherichia coli, Salmonella,
Shigella, and other Enterobacteriaceae, Pseudomonas etc. (Gdoura-Ben Amor et al.
2018) There are 30–40 million bacterial cells in a gram of food. Genus Bacillus
is of prominence especially when taken in consideration the significance for its
role in the biodegradation of fatty materials in food wastes, as it is armed with an
array of important producing capabilities like lipase- and biosurfactant-producing
species, also they are capable of creating endospores that empowers them resistant
to conflicting; extreme environmental situations, hence they have a special niche in
the biological handling of pollutants and xenobiotics. Bacillus spp. along with other
fat-degrading microorganisms like as Acinetobacter spp. and/or Pseudomonas spp.
have been isolated from an assortment of sources, including contaminated food stuffs
S. Swathi Priya · C. Shanmuga Priya · J. Maria Shyla (B)
Department of IT, PSGR Krishnammal College for Women, Coimbatore, TamilNadu, India
e-mail: mariatommy07@gmail.com
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2021
M. Prashanthi et al. (eds.), Bioremediation and Green Technologies, Environmental
Science and Engineering, https://doi.org/10.1007/978-3-030-64122-1_16
235