291
Barley genotypes were sown by hand, 3–5 cm deep, on 18 October in the first
experimental year. In the second testing year, the sowing was done on 14 October.
Pre-sowing soil preparation was conducted in suitable timing, in both years and in
accordance with weather conditions. The barley genotypes were harvested manually in June in both years.
10.2.3 Plant Material
Thirty winter barley genotypes were used as experimental material with different
origin. Six from them are six-row, and the other barley varieties have two-row spike
morphology (Table 10.7). In Table 10.7 are presented the used barley genotypes
with their origin and spike morphology. Five of them are from the Republic of North
Macedonian, five genotypes have Serbian origin, two genotypes are from the
Republic of Croatia, four genotypes are from the Republic of Hungary, two genotypes have Romanian origin, and the remaining ten genotypes have Bulgarian origin
(Table 10.7).
10.2.4 Sample Preparation and Applied Methods
Determination of macro- and microelements was performed in laboratory
“UNILAB,” Faculty of Agriculture, “Goce Delchev” University of Štip, Republic of
North Macedonia. For digestion of barley grain samples, the microwave digestion
system (model Mars, CEM) was applied (Fig. 10.2). Precisely 0.5 g of grind barley
grain was measured and placed in Teflon digestion vessels from each genotype.
5 ml concentrated nitric acid, HNO (69%, 108 m/V), and 2 ml hydrogen peroxide,
Table 10.6 Average air temperature and precipitation sum during the experimental period
Month
Average temperature (°C)
Precipitation sum (mm)
2012–2013
2013–2014
2012–2013
2013–2014
October
16.6
13.0
40.6
11.8
November
10.5
9.1
37.4
65.8
December
1.5
0.3
33.7
20.3
January
2.4
3.9
33.6
42.1
February
15.3
7.0
119.3
12.8
March
9.1
9.6
35.0
25.0
April
13.2
12.2
63.2
148.2
May
18.6
16.6
44.8
59.3
June
21.7
20.3
23.8
69.3
Average
12.1
10.2
431.4
454.6
10 Chemometric Determination of Macro- and Microelements in Barley Genotypes…
Barley genotypes were sown by hand, 3–5 cm deep, on 18 October in the first
experimental year. In the second testing year, the sowing was done on 14 October.
Pre-sowing soil preparation was conducted in suitable timing, in both years and in
accordance with weather conditions. The barley genotypes were harvested manually in June in both years.
10.2.3 Plant Material
Thirty winter barley genotypes were used as experimental material with different
origin. Six from them are six-row, and the other barley varieties have two-row spike
morphology (Table 10.7). In Table 10.7 are presented the used barley genotypes
with their origin and spike morphology. Five of them are from the Republic of North
Macedonian, five genotypes have Serbian origin, two genotypes are from the
Republic of Croatia, four genotypes are from the Republic of Hungary, two genotypes have Romanian origin, and the remaining ten genotypes have Bulgarian origin
(Table 10.7).
10.2.4 Sample Preparation and Applied Methods
Determination of macro- and microelements was performed in laboratory
“UNILAB,” Faculty of Agriculture, “Goce Delchev” University of Štip, Republic of
North Macedonia. For digestion of barley grain samples, the microwave digestion
system (model Mars, CEM) was applied (Fig. 10.2). Precisely 0.5 g of grind barley
grain was measured and placed in Teflon digestion vessels from each genotype.
5 ml concentrated nitric acid, HNO (69%, 108 m/V), and 2 ml hydrogen peroxide,
Table 10.6 Average air temperature and precipitation sum during the experimental period
Month
Average temperature (°C)
Precipitation sum (mm)
2012–2013
2013–2014
2012–2013
2013–2014
October
16.6
13.0
40.6
11.8
November
10.5
9.1
37.4
65.8
December
1.5
0.3
33.7
20.3
January
2.4
3.9
33.6
42.1
February
15.3
7.0
119.3
12.8
March
9.1
9.6
35.0
25.0
April
13.2
12.2
63.2
148.2
May
18.6
16.6
44.8
59.3
June
21.7
20.3
23.8
69.3
Average
12.1
10.2
431.4
454.6
10 Chemometric Determination of Macro- and Microelements in Barley Genotypes…