mentally validated binding to the promoter
regions of UPR-regulated genes identified similar UPREs in S. cerevisiae (Fordyce et al. 2012;
Kohno et al. 1993; Mori et al. 1992), Aspergillus
niger (Mulder et al. 2006), and U. maydis (Pinter et al. 2019) (Fig. 3.3b), the latter of which is
Fig. 3.3 Conservation of Hac1 and UPREs in fungi. (a)
Alignment of bZIP domains of Hac1-like proteins in
various fungi. Cg Colletotrichum graminicola, Tr Trichoderma reesei, Nc Neurospora crassa, Fo Fusarium
oxysporum, Mo Magnaporthe oryzae, Af Aspergillus
fumigatus, Ao Aspergillus oryzae, Anig Aspergillus
niger, An Aspergillus nidulans, Bc Botrytis cinerea, Ab
Alternaria brassicicola, Sc Saccharomyces cerevisiae, Yl
Yarrowia lipolytica, Ca Candida albicans, Um Ustilago
maydis, Lb Laccaria bicolor, Cc Coprinopsis cinerea, Cn
Cryptococcus neoformans, Hs Homo sapiens. (b) Experimentally verified UPRE motifs bound by Hac1-like
proteins in indicated fungi. (c) Phylogenetic relationship between Hac1-like proteins. The phylogenetic tree
is based on multiple alignments of the bZIP domains in
Hac1-like proteins from indicated species using ClustalW. Cg Colletotrichum graminicola, Vd Verticillium
dahliae, Tr Trichoderma reesei, Nc Neurospora crassa,
Fo Fusarium oxysporum, Mo Magnaporthe oryzae, Af
Aspergillus fumigatus, Ao Aspergillus oryzae, Anig
Aspergillus niger, An Aspergillus nidulans, Bc Botrytis
cinerea, Ab Alternaria brassicicola, Sc Saccharomyces
cerevisiae, Yl Yarrowia lipolytica, Ca Candida albicans,
Um Ustilago maydis, Lb Laccaria bicolor, Cc Coprinopsis cinerea, Cn Cryptococcus neoformans, Hs Homo
sapiens
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R. Harting and K. Heimel