Ko ¨nig CC, Scherlach K, Schroeckh V et al (2013) Bacterium induces cryptic meroterpenoid pathway in
the pathogenic fungus Aspergillus fumigatus.
ChemBioChem
14:938–942.
https://doi.org/
10.1002/cbic.201300070
Krappmann S (2017) CRISPR-Cas9, the new kid on the
block of fungal molecular biology. Med Mycol
55:16–23. https://doi.org/10.1093/mmy/myw097
Kumla D, Pereira J, Dethoup T et al (2018) Chromone
derivatives and other constituents from cultures of
the marine sponge-associated fungus Penicillium
erubescens KUFA0220 and their antibacterial
activity. Mar Drugs 16:289. https://doi.org/
10.3390/md16080289
Lei H, Lin X, Han L et al (2017) New metabolites and
bioactive chlorinated benzophenone derivatives
produced by a marine-derived fungus Pestalotiopsis heterocornis. Mar Drugs 15:69. https://doi.org/
10.3390/md15030069
Ley A, Coumou HC, Frandsen RJN (2015) Heterologous
expression of MlcE in Saccharomyces cerevisiae
provides resistance to natural and semi-synthetic
statins. Metab Eng Commun 2:117–123. https://
doi.org/10.1016/j.meteno.2015.09.003
Li C, Wang J, Luo C et al (2014) A new cyclopeptide
with antifungal activity from the co-culture broth
of two marine mangrove fungi. Nat Prod Res
28:616–621.
https://doi.org/10.1080/
14786419.2014.887074
Lin H, Lyu H, Zhou S et al (2018) Deletion of a global
regulator LaeB leads to the discovery of novel
polyketides in Aspergillus nidulans. Org Biomol
Chem 16:4973–4976. https://doi.org/10.1039/
C8OB01326H
Liu X, Locasale JW (2017) Metabolomics: a primer.
Trends Biochem Sci 42:274–284. https://doi.org/
10.1016/j.tibs.2017.01.004
Liu R, Chen L, Jiang Y et al (2015) Efficient genome
editing in filamentous fungus Trichoderma reesei
using the CRISPR/Cas9 system. Cell Discov
1:15007. https://doi.org/10.1038/celldisc.2015.7
Liu J, Gu B, Yang L et al (2018) New anti-inflammatory
cyclopeptides from a sponge-derived fungus
Aspergillus violaceofuscus. Front Chem 6:226.
https://doi.org/10.3389/fchem.2018.00226
Lubertozzi D, Keasling JD (2009) Developing Aspergillus as a host for heterologous expression. Biotechnol Adv 27:53–75. https://doi.org/10.1016/j.
biotechadv.2008.09.001
Macheleidt J, Scherlach K, Neuwirth T et al (2015)
Transcriptome analysis of cyclic AMP-dependent
protein kinase A-regulated genes reveals the production of the novel natural compound fumipyrrole by Aspergillus fumigatus. Mol Microbiol
96:148–162. https://doi.org/10.1111/mmi.12926
Macheleidt J, Mattern DJ, Fischer J et al (2016) Regulation and role of fungal secondary metabolites.
Annu Rev Genet 50:371–392. https://doi.org/
10.1146/annurev-genet-120215-035203
Mandelare PE, Adpressa DA, Kaweesa EN et al (2018)
Coculture of two developmental stages of a
marine-derived Aspergillus alliaceus results in the
production of the cytotoxic bianthrone allianthrone A. J Nat Prod 81:1014–1022. https://doi.
org/10.1021/acs.jnatprod.8b00024
Marfori EC, Kajiyama S, Fukusaki E, Kobayashi A
(2002) Trichosetin, a novel tetramic acid antibiotic
produced in dual culture of Trichoderma harzianum and Catharanthus roseus Callus. Z Naturforsch C 57:465–470
Matsu-ura T, Baek M, Kwon J, Hong C (2015) Efficient
gene editing in Neurospora crassa with CRISPR
technology. Fungal Biol Biotechnol 2:4. https://
doi.org/10.1186/s40694-015-0015-1
Mattern DJ, Schoeler H, Weber J et al (2015a) Identification of the antiphagocytic trypacidin gene cluster in the human-pathogenic fungus Aspergillus
fumigatus. Appl Microbiol Biotechnol 99:10151–
10161. https://doi.org/10.1007/s00253-015-6898-1
Mattern DJ, Valiante V, Unkles SE, Brakhage AA
(2015b) Synthetic biology of fungal natural products. Front Microbiol 6:775. https://doi.org/
10.3389/fmicb.2015.00775
Mattern DJ, Valiante V, Horn F et al (2017) Rewiring of
the austinoid biosynthetic pathway in filamentous
fungi. ACS Chem Biol 12:2927–2933. https://doi.
org/10.1021/acschembio.7b00814
Medema MH, Blin K, Cimermancic P et al (2011) antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene
clusters in bacterial and fungal genome sequences.
Nucleic Acids Res 39:W339–W346. https://doi.org/
10.1093/nar/gkr466
Meier JL, Burkart MD (2011) Proteomic analysis of
polyketide and nonribosomal peptide biosynthesis. Curr Opin Chem Biol 15:48–56. https://doi.org/
10.1016/j.cbpa.2010.10.021
Meier JL, Niessen S, Hoover HS et al (2009) An Orthogonal Active Site Identification System (OASIS) for
proteomic profiling of natural product biosynthesis. ACS Chem Biol 4:948–957. https://doi.org/
10.1021/cb9002128
Meng LH, Liu Y, Li XM et al (2015) Citrifelins A and B,
citrinin adducts with a tetracyclic framework from
cocultures of marine-derived isolates of Penicillium citrinum and Beauveria felina. J Nat Prod
78:2301–2305.
https://doi.org/10.1021/acs.jnatprod.5b00450
Meyer V, Wanka F, van Gent J et al (2011) Fungal
gene expression on demand: an inducible, tunable,
and metabolism-independent expression system
for Aspergillus niger. Appl Environ Microbiol
77:2975–2983. https://doi.org/10.1128/AEM.0274010
Milshteyn A, Schneider JS, Brady SF (2014) Mining the
metabiome: identifying novel natural products from
microbial communities. Chem Biol 21:1211–1223.
https://doi.org/10.1016/j.chembiol.2014.08.006
11 New Avenues Toward Drug Discovery in Fungi
291
the pathogenic fungus Aspergillus fumigatus.
ChemBioChem
14:938–942.
https://doi.org/
10.1002/cbic.201300070
Krappmann S (2017) CRISPR-Cas9, the new kid on the
block of fungal molecular biology. Med Mycol
55:16–23. https://doi.org/10.1093/mmy/myw097
Kumla D, Pereira J, Dethoup T et al (2018) Chromone
derivatives and other constituents from cultures of
the marine sponge-associated fungus Penicillium
erubescens KUFA0220 and their antibacterial
activity. Mar Drugs 16:289. https://doi.org/
10.3390/md16080289
Lei H, Lin X, Han L et al (2017) New metabolites and
bioactive chlorinated benzophenone derivatives
produced by a marine-derived fungus Pestalotiopsis heterocornis. Mar Drugs 15:69. https://doi.org/
10.3390/md15030069
Ley A, Coumou HC, Frandsen RJN (2015) Heterologous
expression of MlcE in Saccharomyces cerevisiae
provides resistance to natural and semi-synthetic
statins. Metab Eng Commun 2:117–123. https://
doi.org/10.1016/j.meteno.2015.09.003
Li C, Wang J, Luo C et al (2014) A new cyclopeptide
with antifungal activity from the co-culture broth
of two marine mangrove fungi. Nat Prod Res
28:616–621.
https://doi.org/10.1080/
14786419.2014.887074
Lin H, Lyu H, Zhou S et al (2018) Deletion of a global
regulator LaeB leads to the discovery of novel
polyketides in Aspergillus nidulans. Org Biomol
Chem 16:4973–4976. https://doi.org/10.1039/
C8OB01326H
Liu X, Locasale JW (2017) Metabolomics: a primer.
Trends Biochem Sci 42:274–284. https://doi.org/
10.1016/j.tibs.2017.01.004
Liu R, Chen L, Jiang Y et al (2015) Efficient genome
editing in filamentous fungus Trichoderma reesei
using the CRISPR/Cas9 system. Cell Discov
1:15007. https://doi.org/10.1038/celldisc.2015.7
Liu J, Gu B, Yang L et al (2018) New anti-inflammatory
cyclopeptides from a sponge-derived fungus
Aspergillus violaceofuscus. Front Chem 6:226.
https://doi.org/10.3389/fchem.2018.00226
Lubertozzi D, Keasling JD (2009) Developing Aspergillus as a host for heterologous expression. Biotechnol Adv 27:53–75. https://doi.org/10.1016/j.
biotechadv.2008.09.001
Macheleidt J, Scherlach K, Neuwirth T et al (2015)
Transcriptome analysis of cyclic AMP-dependent
protein kinase A-regulated genes reveals the production of the novel natural compound fumipyrrole by Aspergillus fumigatus. Mol Microbiol
96:148–162. https://doi.org/10.1111/mmi.12926
Macheleidt J, Mattern DJ, Fischer J et al (2016) Regulation and role of fungal secondary metabolites.
Annu Rev Genet 50:371–392. https://doi.org/
10.1146/annurev-genet-120215-035203
Mandelare PE, Adpressa DA, Kaweesa EN et al (2018)
Coculture of two developmental stages of a
marine-derived Aspergillus alliaceus results in the
production of the cytotoxic bianthrone allianthrone A. J Nat Prod 81:1014–1022. https://doi.
org/10.1021/acs.jnatprod.8b00024
Marfori EC, Kajiyama S, Fukusaki E, Kobayashi A
(2002) Trichosetin, a novel tetramic acid antibiotic
produced in dual culture of Trichoderma harzianum and Catharanthus roseus Callus. Z Naturforsch C 57:465–470
Matsu-ura T, Baek M, Kwon J, Hong C (2015) Efficient
gene editing in Neurospora crassa with CRISPR
technology. Fungal Biol Biotechnol 2:4. https://
doi.org/10.1186/s40694-015-0015-1
Mattern DJ, Schoeler H, Weber J et al (2015a) Identification of the antiphagocytic trypacidin gene cluster in the human-pathogenic fungus Aspergillus
fumigatus. Appl Microbiol Biotechnol 99:10151–
10161. https://doi.org/10.1007/s00253-015-6898-1
Mattern DJ, Valiante V, Unkles SE, Brakhage AA
(2015b) Synthetic biology of fungal natural products. Front Microbiol 6:775. https://doi.org/
10.3389/fmicb.2015.00775
Mattern DJ, Valiante V, Horn F et al (2017) Rewiring of
the austinoid biosynthetic pathway in filamentous
fungi. ACS Chem Biol 12:2927–2933. https://doi.
org/10.1021/acschembio.7b00814
Medema MH, Blin K, Cimermancic P et al (2011) antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene
clusters in bacterial and fungal genome sequences.
Nucleic Acids Res 39:W339–W346. https://doi.org/
10.1093/nar/gkr466
Meier JL, Burkart MD (2011) Proteomic analysis of
polyketide and nonribosomal peptide biosynthesis. Curr Opin Chem Biol 15:48–56. https://doi.org/
10.1016/j.cbpa.2010.10.021
Meier JL, Niessen S, Hoover HS et al (2009) An Orthogonal Active Site Identification System (OASIS) for
proteomic profiling of natural product biosynthesis. ACS Chem Biol 4:948–957. https://doi.org/
10.1021/cb9002128
Meng LH, Liu Y, Li XM et al (2015) Citrifelins A and B,
citrinin adducts with a tetracyclic framework from
cocultures of marine-derived isolates of Penicillium citrinum and Beauveria felina. J Nat Prod
78:2301–2305.
https://doi.org/10.1021/acs.jnatprod.5b00450
Meyer V, Wanka F, van Gent J et al (2011) Fungal
gene expression on demand: an inducible, tunable,
and metabolism-independent expression system
for Aspergillus niger. Appl Environ Microbiol
77:2975–2983. https://doi.org/10.1128/AEM.0274010
Milshteyn A, Schneider JS, Brady SF (2014) Mining the
metabiome: identifying novel natural products from
microbial communities. Chem Biol 21:1211–1223.
https://doi.org/10.1016/j.chembiol.2014.08.006
11 New Avenues Toward Drug Discovery in Fungi
291